Video-rate wide-field coherent anti-Stokes Raman scattering microscopy with collinear nonphase-matching illumination

Ming Lei; Martin Winterhalder; Romedi Selm; Andreas Zumbusch

doi:10.1117/1.3533707

1 February 2011 Video-rate wide-field coherent anti-Stokes Raman scattering microscopy with collinear nonphase-matching illumination

Ming Lei, Martin Winterhalder, Romedi Selm, Andreas Zumbusch

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Journal of Biomedical Optics, Vol. 16, Issue 2, 021102 (February 2011). https://doi.org/10.1117/1.3533707

Abstract

A simple scheme for video-rate wide-field coherent anti-Stokes Raman scattering (CARS) microscopy is presented. The method is based on collinear nonphase-matching illumination. The mechanisms leading to CARS signal generation are investigated. We find that refraction-mediated phase-matching is the main effect. Video-rate wide-field CARS microscopy of polystyrene beads and CARS wide-field images of C. elegans embryos are shown, and the capabilities and the limitations of the scheme are discussed.

©(2011) Society of Photo-Optical Instrumentation Engineers (SPIE)

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Ming Lei, Martin Winterhalder, Romedi Selm, and Andreas Zumbusch "Video-rate wide-field coherent anti-Stokes Raman scattering microscopy with collinear nonphase-matching illumination," Journal of Biomedical Optics 16(2), 021102 (1 February 2011). https://doi.org/10.1117/1.3533707

Published: 1 February 2011

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