Pulse duration determines levels of Hsp70 induction in tissues following laser irradiation

Mark A. Mackanos; Christopher H. Contag

doi:10.1117/1.3600013

1 July 2011 Pulse duration determines levels of Hsp70 induction in tissues following laser irradiation

Mark A. Mackanos, Christopher H. Contag

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Journal of Biomedical Optics, Vol. 16, Issue 7, 078002 (July 2011). https://doi.org/10.1117/1.3600013

Abstract

Induction of heat shock protein (Hsp) expression correlates with cytoprotection, reduced tissue damage, and accelerated healing in animal models. Since Hsps are transcriptionally activated in response to stress, they can act as stress indicators in burn injury or surgical procedures that produce heat and thermal change. A fast in vivo readout for induction of Hsp transcription in tissues would allow for the study of these proteins as therapeutic effect mediators and reporters of thermal stress/damage. We used a transgenic reporter mouse in which a luciferase expression is controlled by the regulatory region of the inducible 70 kilodalton (kDa) Hsp as a rapid readout of cellular responses to laser-mediated thermal stress/injury in mouse skin. We assessed the pulse duration dependence of the Hsp70 expression after irradiation with a CO2 laser at 10.6 μm in wavelength over a range of 1000 to 1 ms. Hsp70 induction varied with changes in laser pulse durations and radiant exposures, which defined the ranges at which thermal activation of Hsp70 can be used to protect cells from subsequent stress, and reveals the window of thermal stress that tissues can endure.

©(2011) Society of Photo-Optical Instrumentation Engineers (SPIE)

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Mark A. Mackanos and Christopher H. Contag "Pulse duration determines levels of Hsp70 induction in tissues following laser irradiation," Journal of Biomedical Optics 16(7), 078002 (1 July 2011). https://doi.org/10.1117/1.3600013

Published: 1 July 2011

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