Spectrally resolved multidepth fluorescence imaging

Yuan Luo; Ioannis K. Zervantonakis; Se Baek Oh; Roger D. Kamm; George Barbastathis

doi:10.1117/1.3626211

1 September 2011 Spectrally resolved multidepth fluorescence imaging

Yuan Luo, Ioannis K. Zervantonakis, Se Baek Oh, Roger D. Kamm, George Barbastathis

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Journal of Biomedical Optics, Vol. 16, Issue 9, 096015 (September 2011). https://doi.org/10.1117/1.3626211

Abstract

We present a multicolor fluorescence imaging modality to visualize in real-time tissue structures emitting multispectral fluorescent light from different focal depths. Each designated spectrum of fluorescent emission from a specific depth within a volumetric tissue is probed by a depth-spectrum selective holographic grating. The grating for each fluorescent color are multiplexed within a volume hologram, which enables simultaneously obtaining multicolored fluorescent information at different depths within a biological tissue sample. We demonstrate the imaging modality's ability to obtain laser-induced multicolored fluorescence images of a biological sample from different depths without scanning. We also experimentally demonstrate that the imaging modality can be simultaneously operated at both fluorescent and bright field modes to provide complementary information of volumetric tissue structures at different depths in real-time.

©(2011) Society of Photo-Optical Instrumentation Engineers (SPIE)

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Yuan Luo, Ioannis K. Zervantonakis, Se Baek Oh, Roger D. Kamm, and George Barbastathis "Spectrally resolved multidepth fluorescence imaging," Journal of Biomedical Optics 16(9), 096015 (1 September 2011). https://doi.org/10.1117/1.3626211

Published: 1 September 2011

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