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26 March 2012 Autofocus evaluation for brightfield microscopy pathology
Rafael Redondo, Gabriel Cristóbal, Gloria Bueno Garcia, Oscar Deniz, Jesus Salido, Maria del Milagro Fernandez, Juan Vidal, Juan Carlos Valdiviezo, Rodrigo Nava, Boris Escalante-Ramírez, Marcial Garcia-Rojo
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Journal of Biomedical Optics, Vol. 17, Issue 3, 036008 (March 2012). https://doi.org/10.1117/1.JBO.17.3.036008
Abstract
An essential and indispensable component of automated microscopy framework is the automatic focusing system, which determines the in-focus position of a given field of view by searching the maximum value of a focusing function over a range of z-axis positions. The focus function and its computation time are crucial to the accuracy and efficiency of the system. Sixteen focusing algorithms were analyzed for histological and histopathological images. In terms of accuracy, results have shown an overall high performance by most of the methods. However, we included in the evaluation study other criteria such as computational cost and focusing curve shape which are crucial for real-time applications and were used to highlight the best practices.
© 2012 Society of Photo-Optical Instrumentation Engineers (SPIE) 0091-3286/2012/$25.00 © 2012 SPIE
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Rafael Redondo, Gabriel Cristóbal, Gloria Bueno Garcia, Oscar Deniz, Jesus Salido, Maria del Milagro Fernandez, Juan Vidal, Juan Carlos Valdiviezo, Rodrigo Nava, Boris Escalante-Ramírez, and Marcial Garcia-Rojo "Autofocus evaluation for brightfield microscopy pathology," Journal of Biomedical Optics 17(3), 036008 (26 March 2012). https://doi.org/10.1117/1.JBO.17.3.036008
Published: 26 March 2012
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Cited by 57 scholarly publications and 2 patents.
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KEYWORDS
Microscopy
Pathology
Cell biology
Tissues
Biopsy
Breast
Image analysis
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