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4 May 2012 Optimal ultraviolet wavelength for in vivo photoacoustic imaging of cell nuclei
Da-Kang Yao, Konstantin I. Maslov, Lihong V. Wang, Ruimin Chen, Qifa Zhou
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Journal of Biomedical Optics, Vol. 17, Issue 5, 056004 (May 2012). https://doi.org/10.1117/1.JBO.17.5.056004
Abstract
In order to image noninvasively cell nuclei in vivo without staining, we have developed ultraviolet photoacoustic microscopy (UV-PAM), in which ultraviolet light excites nucleic acids in cell nuclei to produce photoacoustic waves. Equipped with a tunable laser system, the UV-PAM was applied to in vivo imaging of cell nuclei in small animals. We found that 250 nm was the optimal wavelength for in vivo photoacoustic imaging of cell nuclei. The optimal wavelength enables UV-PAM to image cell nuclei using as little as 2 nJ laser pulse energy. Besides the optimal wavelength, application of a wavelength between 245 and 275 nm can produce in vivo images of cell nuclei with specific, positive, and high optical contrast.
© 2012 Society of Photo-Optical Instrumentation Engineers (SPIE) 0091-3286/2012/$25.00 © 2012 SPIE
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Da-Kang Yao, Konstantin I. Maslov, Lihong V. Wang, Ruimin Chen, and Qifa Zhou "Optimal ultraviolet wavelength for in vivo photoacoustic imaging of cell nuclei," Journal of Biomedical Optics 17(5), 056004 (4 May 2012). https://doi.org/10.1117/1.JBO.17.5.056004
Published: 4 May 2012
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Cited by 65 scholarly publications and 9 patents.
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KEYWORDS
In vivo imaging
Skin
Photoacoustic spectroscopy
Ultraviolet radiation
Ear
Photoacoustic imaging
Pulsed laser operation
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