CONFERENCE PROCEEDINGS
Advanced Search
Home > Journals > J. Biomed. Opt. > Volume 17 > Issue 7 > Article
Open Access
14 June 2012 Three-dimensional simultaneous optical coherence tomography and confocal fluorescence microscopy for investigation of lung tissue
Maria Gärtner, Peter Cimalla, Sven Meissner, Edmund Koch, Wolfgang Michael Kübler
Author Affiliations +
Journal of Biomedical Optics, Vol. 17, Issue 7, 071310 (June 2012). https://doi.org/10.1117/1.JBO.17.7.071310
Abstract
Although several strategies exist for a minimal-invasive treatment of patients with lung failure, the mortality rate of acute respiratory distress syndrome still reaches 30% at minimum. This striking number indicates the necessity of understanding lung dynamics on an alveolar level. To investigate the dynamical behavior on a microscale, we used three-dimensional geometrical and functional imaging to observe tissue parameters including alveolar size and length of embedded elastic fibers during ventilation. We established a combined optical coherence tomography (OCT) and confocal fluorescence microscopy system that is able to monitor the distension of alveolar tissue and elastin fibers simultaneously within three dimensions. The OCT system can laterally resolve a 4.9 μm line pair feature and has an approximately 11 μm full-width-half-maximum axial resolution in air. confocal fluorescence microscopy visualizes molecular properties of the tissue with a resolution of 0.75 μm (laterally), and 5.9 μm (axially) via fluorescence detection of the dye sulforhodamine B specifically binding to elastin. For system evaluation, we used a mouse model in situ to perform lung distension by application of different constant pressure values within the physiological regime. Our method enables the investigation of alveolar dynamics by helping to reveal basic processes emerging during artificial ventilation and breathing.
© 2012 Society of Photo-Optical Instrumentation Engineers (SPIE) 0091-3286/2012/$25.00 © 2012 SPIE
Citation Download Citation
Maria Gärtner, Peter Cimalla, Sven Meissner, Edmund Koch, and Wolfgang Michael Kübler "Three-dimensional simultaneous optical coherence tomography and confocal fluorescence microscopy for investigation of lung tissue," Journal of Biomedical Optics 17(7), 071310 (14 June 2012). https://doi.org/10.1117/1.JBO.17.7.071310
Published: 14 June 2012
JOURNAL ARTICLE
 9 PAGES
DOWNLOAD PAPER SAVE TO MY LIBRARY
GET CITATION
Lens.org Logo
CITATIONS
Cited by 14 scholarly publications.
Advertisement
Advertisement
RIGHTS & PERMISSIONS
Get copyright permission  Get copyright permission on Copyright Marketplace
KEYWORDS
Optical coherence tomography
Luminescence
Lung
Optical fibers
Confocal microscopy
Tissues
Microscopy
RELATED CONTENT
Subscribe to Digital Library
Receive Erratum Email Alert
Back to Top