Fluorescence Talbot microscope using incoherent source

Yangyang Sun; Shuo Pang

doi:10.1117/1.JBO.21.8.086003

8 August 2016 Fluorescence Talbot microscope using incoherent source

Yangyang Sun, Shuo Pang

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Journal of Biomedical Optics, Vol. 21, Issue 8, 086003 (August 2016). https://doi.org/10.1117/1.JBO.21.8.086003

Abstract

Fluorescence Talbot microscope is a scalable field-of-view (FOV) imaging platform, which takes advantage of the phase sensitivity of the self-image of a periodic structure. Such a system can maintain the microscopic resolution and extend the FOV for the whole slide (15 mm×15 mm) scanning. Previously reported Talbot fluorescence systems, tabletop and on-chip device alike, rely on the coherence of the illumination source, limiting their potential applications in low-resource setting environment. A more cost-effective setup using a light-emitting diode, which has an area of 4 mm² and a full width at half maximum of 16 nm in wavelength, is demonstrated. Compared to the illumination that is spatially filtered by a single pinhole, our system has achieved an illumination intensity that is 357 times higher. The reconstructed image quality is comparable to that of a 10× microscope objective. Various samples, such as fluorescent beads, green fluorescence protein-labeled HeLa cells, and a mouse kidney slide, were reconstructed by the system.

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Yangyang Sun and Shuo Pang "Fluorescence Talbot microscope using incoherent source," Journal of Biomedical Optics 21(8), 086003 (8 August 2016). https://doi.org/10.1117/1.JBO.21.8.086003

Published: 8 August 2016

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