Tunable structured illumination light sheet microscopy for background rejection and imaging depth in minimally processed tissues

Joseph R. Landry; Ryosuke Itoh; Jonathan M. Li; Stephen S. Hamann; Michael Mandella; Christopher H. Contag; Olav Solgaard

doi:10.1117/1.JBO.24.4.046501

9 April 2019 Tunable structured illumination light sheet microscopy for background rejection and imaging depth in minimally processed tissues

Joseph R. Landry, Ryosuke Itoh, Jonathan M. Li, Stephen S. Hamann, Michael Mandella, Christopher H. Contag, Olav Solgaard

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Journal of Biomedical Optics, Vol. 24, Issue 4, 046501 (April 2019). https://doi.org/10.1117/1.JBO.24.4.046501

Abstract

We demonstrate improved optical sectioning in light sheet fluorescence microscopy using tunable structured illumination (SI) frequencies to optimize image quality in scattering specimens. The SI patterns are generated coherently using a one-dimensional spatial light modulator for maximum pattern contrast, and the pattern spatial frequency is adjustable up to half the incoherent cutoff frequency of our detection objective. At this frequency, we demonstrate background reductions of 2 orders of magnitude.

CC BY: © The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

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Joseph R. Landry, Ryosuke Itoh, Jonathan M. Li, Stephen S. Hamann, Michael Mandella, Christopher H. Contag, and Olav Solgaard "Tunable structured illumination light sheet microscopy for background rejection and imaging depth in minimally processed tissues," Journal of Biomedical Optics 24(4), 046501 (9 April 2019). https://doi.org/10.1117/1.JBO.24.4.046501

Received: 2 January 2019; Accepted: 22 March 2019; Published: 9 April 2019

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