1.Introduction1.1.Necrotizing Soft-Tissue InfectionsNecrotizing soft-tissue infections (NSTIs) are aggressive infections with a cumulative case fatality rate near 21%1 (range, 6% to 76%).2–9 An NSTI occurs when highly virulent, exotoxin-producing bacteria are introduced to muscle, fascia, or subcutaneous tissue from penetrating wounds or following relatively minor, non-penetrating soft tissue injuries. NSTIs may be polymicrobial (i.e., caused by mixed aerobic/anaerobic pathogens) or monomicrobial (i.e., caused by a single pathogen such as group A Streptococcus, clostridial species, and others). Risk factors include blunt trauma, surgical incisions, insect bites, superficial cuts and abrasions, injection sites, muscle strains, pre-existing ulcers or fistulas, peri-rectal abscesses, burns, splinters, chicken pox, childbirth, penetrating injuries, diabetes, and other immunosuppressing conditions.6,9–15 Disruption of the barrier function provides microbial access to deeper soft tissues, though not all patients have such defined portals of bacterial entry, which makes them at higher risk for delayed diagnosis and treatment. Bacterial proliferation with concomitant exotoxin production leads to rapid disease progression, often within hours—causing widespread destruction of muscle, fascia, and skin; sepsis; multi-organ failure; and death. People of all ages and backgrounds can contract an NSTI, including otherwise healthy individuals, children, and neonates.8,10,11,15 The extreme virulence, morbidity, and mortality of NSTIs derive from the ability of causative bacteria to dysregulate microvascular function leading to irreversible tissue perfusion deficits9 and to commandeer the body’s acute-phase immune response such that the infection circumvents the body’s typical containment mechanisms against the spread of infection.16 The emergent nature and relatively low prevalence of NSTI (0.3 to 15.5 cases per 100,000 people, depending on the geographical location) make studying these infections and establishing advancements in care challenging.9 1.2.Standard-of-Care for NSTIsAt present, standard-of-care management for NSTIs is twofold: (1) immediate, broad-spectrum intravenous (IV) antibiotics and (2) emergent surgical debridement of affected tissues. Surgical debridement aims to remove all causative bacteria without concern for the preservation of important structures, such as major blood vessels and nerves.13 In cases of NSTI affecting an extremity, amputation is required in 19% to 26% of cases.1,17 Multiple factors determine the likelihood of survival with an NSTI; the most critical is rapid diagnosis and emergent initiation of treatment—broad-spectrum IV antibiotics and surgical debridement.1,6 Based on a meta-analysis of 16 NSTI clinical studies, surgical treatment within 12 h of presentation resulted in a statistically significant reduction in mortality (19% versus 34% for delayed surgical treatment).1 1.3.Challenge of Real-Time Diagnosis: Exams, Imaging, Frozen Section Pathology, and the LRINEC ScoreReal-time NSTI diagnosis is challenging and often delayed because patients commonly present with non-specific signs and symptoms (e.g., fever, pain, superficial erythema, and elevated inflammatory laboratory values), especially among patients lacking a defined portal of bacterial entry. Therefore, NSTIs may easily be missed initially or mistaken for other superficial infections, such as cellulitis, which are less emergent and are usually treated with antibiotics alone.18 In two series, only 15% to 25% of patients were diagnosed correctly at the time of presentation; 75% to 85% of patients were classified as having a delay in diagnosis.6,19 Delays in management allow bacteria to proliferate and advance centrally, quickly leading to sepsis, irreversible organ failure, and ultimately death. Because of the high stakes of ascertaining a diagnosis of NSTI, clinical investigators have evaluated numerous strategies for NSTI diagnosis, including vital signs, physical examination, radiological imaging, frozen section histology, and clinical scoring systems.9,20,21 Vital signs and physical examination are non-specific for NSTI, with little diagnostic accuracy (Table S1 in the Supplementary Material).10,22–24 The presence of bullae, ecchymoses, and hypotension have high specificity for NSTIs; however, these are late indicators of tissue destruction, are associated with high mortality, and are therefore unsuited for early NSTI diagnosis.10,23,25,26 Radiographs (i.e., projection X-ray imaging) and computed tomography (CT) are used routinely to evaluate patients with suspected NSTIs. Pooled specificities for radiographs and CT are 91% and 95%, respectively, but pooled sensitivities are marginal for both imaging modalities (62% for radiography and 71% for CT; results based on a meta-analysis of four radiography and seven CT studies; Table S1 in the Supplementary Material).20,27–29 Magnetic resonance imaging (MRI) provides higher pooled sensitivity (86%) but lower pooled specificity (65%) based on a recent meta-analysis of six studies.30 Consensus guidelines for CT-based or MRI-based NSTI diagnosis have not been established to date. In addition, the time requirements for imaging, particularly MRI, lead to delays in care.31 Tissue biopsy with frozen section histology was initially reported to be an accurate method of diagnosis,21,32 but more recent clinical study results report it to be less reliable, with low sensitivity (32%).29 The Laboratory Risk Indicator for Necrotizing Fasciitis (LRINEC) score is the most highly cited and evaluated tool for identifying NSTIs and is based on laboratory test results (see Table S2 in the Supplementary Material).22 However, the LRINEC score yields marginal overall diagnostic performance, particularly sensitivity. A meta-analysis of 23 clinical studies of adult NSTI patients reported pooled sensitivity and specificity of 68% and 85%, respectively, using the standard cutoff. Using the same dataset, if had alternatively been used for diagnosis, pooled sensitivity and specificity were 41% and 95%, respectively.20 Furthermore, relatively few studies have prospectively evaluated the scoring system for NSTI identification.28,33,34 The gold standard for diagnosis of NSTIs requires concordant tissue culture and permanent section histopathology,29 both of which require invasive testing (i.e., tissue biopsy and therefore a high degree of suspicion) and several days to provide a result. No current strategies yield an accurate, real-time diagnosis. 1.4.Fluorescence Guidance with Indocyanine GreenFluorescence-guided surgery (FGS) is a rapidly evolving and growing field focused on improving surgical efficacy and safety through enhanced visual recognition of critical structures (e.g., tumors, nerves, blood vessels) or physiological phenomena (e.g., enzymatic activity and vascular/luminal patency). FGS utilizes fluorescent probes (fluorophores) with varied mechanisms for tissue identification, including non-targeted intravascular/intraluminal perfusion probes, molecular-targeted probes, and probes activated by enzymatic or metabolic activity. Today, multiple FGS imaging systems are Food and Drug Administration (FDA)-approved for clinical use or are in trials.35–38 Indocyanine green (ICG) is a non-targeted, intravascular, near-infrared fluorophore with an excellent safety record and years of FDA approval for angiography, ophthalmology, and liver function assessment.39 ICG has a peak spectral absorption at and peak spectral emission at in blood.40 Within seconds of IV administration, ICG enables the visual demarcation of local vasculature, distinguishing perfused tissues from non-perfused tissues. ICG dynamic contrast-enhanced fluorescence imaging (DCE-FI) involves collecting fluorescence image videos for seconds to minutes to measure perfusion first-pass kinetic parameters in target tissues.41–43 ICG fluorescence imaging for the management of NSTI was first reported by Aka et al.44 in 2021. They used ICG fluorescence to distinguish between viable and nonviable tissues to guide repeat surgical debridement in a single NSTI case. The present study advances on the initial report by Aka et al.44 in the following ways: (1) hypothesizing how NSTI pathophysiology at the histological level impacts perfusion and enables ICG DCE-FI as a potential diagnostic tool; (2) imaging patients with NSTI in a cohort-control design; and (3) analyzing fluorescence imaging data using an established DCE-FI technique. A distinguishing histological feature of NSTIs is widespread, microvascular thrombosis—occlusion of capillaries and smaller blood vessels within the soft tissues—initiated by the effects of bacterial exotoxins on platelets, leukocytes, and endothelial cells45,46 and further promulgated by NSTI-activated endothelial cell production of tissue factor.47 We hypothesized that detectable fluorescence signal voids would occur in NSTI-affected tissues following the administration and imaging of ICG fluorescence. Herein, we report recent results from a clinical pilot study to test this hypothesis. 2.Methods2.1.Study DesignAll aspects of this first-in-kind pilot study adhered to a Dartmouth Health Institutional Review Board-approved protocol. The study (ClinicalTrials.gov identifier: NCT04839302) followed a prospective, non-randomized, observational design to test the hypothesis that measurable fluorescence signal voids occur in NSTI-affected tissues following IV administration and imaging of ICG fluorescence (Fig. 1). Patients presenting to the Emergency Department (ED) at Dartmouth Hitchcock Medical Center, a tertiary care academic hospital in Lebanon, New Hampshire, who were suspected of having an NSTI were identified by ED providers. ED providers notified study team members. Study team members educated and consented eligible patients. Inclusion criteria were as follows: (1) years; (2) clinical suspicion of NSTI warranting hospital admission for (a) observation due to suspected NSTI, (b) soft-tissue biopsy, and/or (c) surgical debridement; and (3) ability to give written informed consent (or have legal decision maker present). Exclusion criteria were as follows: (1) history of allergy to ICG and/or iodine48 and (2) pregnant women or nursing mothers. Systemic ICG is cleared by the liver and excreted via bile.49 Thus, liver function impacts ICG perfusion kinetics, particularly over longer time lengths (i.e., 10s of minutes or more). Liver failure was not considered an exclusion criterion in this study but was retrospectively documented for all patients enrolled in the study. Enrolled patients received an IV dose of ICG and were imaged using a portable, cart-based, open-field, FDA-approved imager (a SPY Elite or SPY PHI, Stryker, Kalamazoo, Michigan, United States). The imaged field of view was centered on the region of tissue with visible signs of infection (e.g., inflammation, tissue damage). Initial ICG dosing of resulted in detector saturation. Dose reduction to consistently yielded full dynamic range imaging with minimal saturation. Fluorescence imaging at 4 to 30 frames per second occurred for prior to IV administration of ICG and continued for post-administration to measure first-pass perfusion kinetics in the affected and adjacent tissues. A working distance of was maintained for all cases. In the case of the handheld SPY PHI imager, a three-dimensional (3D)-printed attachment with a laser range finder (see center panel in Fig. 1) was used to maintain the appropriate working distance. DCE-FI analysis was performed “offline” (i.e., not in the clinical theater). The patient engagement did not exceed 10 min per case. After imaging, each patient proceeded with standard-of-care management. The clinical team was blinded to the fluorescence imaging, and imaging did not influence the care delivered to patients. 2.2.Dynamic Contrast-Enhanced Fluorescence ImagingICG fluorescence image data were processed to create wide field-of-view DCE-FI parameter maps using a custom MATLAB script (v2023a, MathWorks, Natick, Massachusetts, United States).41–43 Specifically, ingress slope (IS), time-to-peak (TTP) intensity, and maximum fluorescence intensity (IMAX) were quantified. In addition, a representative, high-contrast, raw fluorescence image (i.e., a “snapshot”) was manually selected from the video recording as a fourth parameter map for analysis. These steps are illustrated in Figs. S1(a)–1(b) in the Supplementary Material. 2.3.Clinical and Image Data AnalysisClinical variables recorded from each patient included basic demographics (age, sex), the presence of comorbidities (diabetes mellitus, peripheral vascular disease), and standard-of-care laboratory blood test results used to derive the LRINEC score (C-reactive protein level, white blood cell count, hemoglobin level, sodium level, creatinine level, and glucose level; see Table S2 in the Supplementary Material for LRINEC scoring system details).22 The ICG fluorescence image data—in the form of the four parameter maps described above (snapshot, IS, TTP, and IMAX)—were further analyzed using custom MATLAB scripts. Tissue pixels were isolated using manually defined regions of interest (ROIs). Using each patient’s representative, high-contrast fluorescence snapshot, ROIs of markedly decreased fluorescence intensity, if present, were delineated by eye and labeled “lesion” ROIs. If no signal voids were present, the tissue region in the center of the field of view was selected as the “lesion” ROI. ROIs of background and lesion tissues were then randomly sampled in pairs to derive signal-to-background ratio (SBR) distributions for all four parameters. These SBR values quantified the contrast to tissues affected by soft tissue infection relative to surrounding tissues within each patient. These steps are illustrated in Fig. S1(c) of the Supplementary Material. 2.4.Statistical AnalysisStatistical analysis was implemented in three steps. First, median values for all clinical variables were statistically evaluated between the NSTI patient group and (1) all non-NSTI patients and (2) only cellulitis patients using the Mann–Whitney test. Second, median parameter map values from lesion ROIs were statistically evaluated between the NSTI patient group and (1) all non-NSTI patients and (2) only cellulitis patients using the Mann–Whitney test. Third, median lesion ROI values were also used for receiver operating characteristic (ROC) curve analysis to quantify the discriminatory power of each parameter for separating NSTI cases from non-NSTI cases. An optimal ROC curve threshold that balanced sensitivity and specificity was used in all cases unless otherwise stated. All statistical tests were implemented using the Statistics and Machine Learning Toolbox (v12.5) in MATLAB. A indicated statistical significance. 3.ResultsA total of 16 patients were enrolled between August 2021 and August 2023. Two of the 16 patients withdrew from the study before data collection; thus, data from 14 patients were analyzed (average age of 57 years; age range of 34 to 78 years; 57% male; Table 1). Six of the 14 patients had confirmed NSTIs (43%), six had cellulitis (43%), and two had other diagnoses (14%; one case of sterile, diabetes mellitus-associated gangrene and one case of osteomyelitis). Comorbidities and laboratory blood test results for the NSTI, non-NSTI, and cellulitis only patient groups are listed in Table 2; none revealed a statistically significant difference between groups. See Table S3 in the Supplementary Material for enrolled patients’ individual laboratory blood test results used to derive LRINEC scores. See Table S4 in the Supplementary Material for enrolled patients’ liver disease status; no enrolled patients suffered from liver failure, a condition known to impact ICG fluorescence perfusion kinetics.49 See Table S5 in the Supplementary Material for standard-of-care imaging performed for each patient as a component of their NSTI workup. See Table S6 in the Supplementary Material for a list of causative pathogens confirmed by tissue biopsy and culture for all NSTI cases. Table 1Enrolled patient demographics.
Table 2Comorbidities and laboratory blood test results for the NSTI, non-NSTI, and cellulitis only patient groups. Statistical test results (p-values) reflect comparisons to the NSTI patient group.
CRP, C-reactive protein; WBC, white blood cell count. White light color images and ICG fluorescence snapshots of confirmed cases of cellulitis, NSTI, and other diagnoses are shown in Figs. 2Fig. 3–4, respectively. All cellulitis cases exhibited increased overall fluorescence intensity without the presence of signal voids in affected tissues, whereas all NSTI cases contained prominent fluorescence signal voids (see yellow lines and arrows in Fig. 3). An ICG-equivalent, 3D-printed fluorescence standard (QUEL Imaging, White River Junction, Vermont, United States) was pictured in some cases [e.g., Fig. 2(b)] but was not available during all imaging sessions. The cellulitis case in Fig. 2(d) had a bulla readily visualized under room light that yielded an ICG fluorescence signal void; this void was not considered a lesion due to clinical context (i.e., blood perfusion is not expected in bullae). The cases with other diagnoses (diabetes mellitus-associated gangrene, osteomyelitis) also exhibited prominent ICG fluorescence signal voids. SBR distributions from paired ROI sampling are summarized in Fig. 5 for all imaged patients. Median lesion-to-background tissue SBRs based on snapshot, IS, TTP, and IMAX parameter maps ranged from 1.1 to 1.5, 1.1 to 3.1, 1.0 to 1.6, and 1.0 to 1.4, respectively, for the cellulitis group. Meanwhile, these ranges were 3.0 to 9.1, 2.2 to 33.8, 1.0 to 7.5, and 1.5 to 12.7, respectively, for the NSTI patient group. See Figs. S2–S4 in the Supplementary Material for all ICG fluorescence parameter maps and ROI selections used to derive SBR values. Fig. S5 in the Supplementary Material shows representative ICG fluorescence intensity time profiles for cases of NSTI, cellulitis, and gangrene; this figure illustrates that measured fluorescence SBRs stabilize within of ICG ingress into tissue vasculature. Parameter map median pixel values from only lesion ROIs were aggregated from the NSTI, non-NSTI, and cellulitis only patient groups. Pooled results are shown in Table 3 (see Table S7 in the Supplementary Material for individual patient median “lesion” ROI parameter map values). Snapshot, IS, and IMAX parameters exhibited statistical significance between the NSTI and cellulitis patient groups. Table 3ICG fluorescence parameter values (mean ± std) based on the lesion region of interest median pixel value from each patient. Parameters include snapshot (a.u.), ingress slope (IS, a.u./s), time-to-peak (TTP, s), and max intensity (IMAX, a.u.). Statistical test results (p-values) reflect comparisons to the NSTI patient group.
The ICG fluorescence parameters summarized in Table 3 were directly compared with the LRINEC score for discriminating NSTI-positive versus NSTI-negative patients via ROC curve analysis. ROC curves are shown in Fig. 6; results comparing all NSTI cases versus all non-NSTI cases are shown in Fig. 6(a), and results comparing all NSTI cases versus only cellulitis cases are shown in Fig. 6(b). Based on the ROC curves shown in Fig. 6, the diagnostic performances of LRINEC scoring and individual ICG fluorescence parameters are summarized in Table 4. Table 4Diagnostic performance of the LRINEC score and ICG fluorescence parameters (values in Table S7 in the Supplementary Material) based on ROC curve analysis shown in Fig. 5. ROC curve optimal cutoff points were selected to balance sensitivity and specificity, except a standard LRINEC score≥6 cutoff was used.22
4.DiscussionResults from this pilot study support the hypothesis that detectable fluorescence signal voids occur in NSTI-affected tissues following the administration of ICG and fluorescence imaging. Patient 07 [Fig. 3(c)] had extensive skin necrosis, which would mandate surgical debridement even without imaging. However, in several cases, imaging revealed tissue perfusion deficits associated with NSTI that were not visualized via white light [Figs. 3(a), 3(b), 3(d), 3(f)]. Meanwhile, all cellulitis cases exhibited increased overall ICG fluorescence, indicative of a hyperemic response in affected tissues (Fig. 2). A fluorescence SBR is considered useful for distinguishing features in open-field FGS.50 As such, all ICG fluorescence snapshot and DCE-FI parameters provide potentially useful contrast that an ED provider or surgeon might use to rapidly identify patients with NSTIs and visualize affected tissues. In the context of NSTI, perfusion-based ICG fluorescence contrast is fundamentally tied to underlying pathophysiology (i.e., microvascular thrombosis). Overall, tissues affected by NSTI exhibited diminished perfusion relative to surrounding tissues and non-necrotizing infections (i.e., cellulitis). This observation was based on lower snapshot and maximum fluorescence intensities and reduced ingress perfusion into affected tissues. Different parameters offered increased SBR depending on the case (Fig. 5), suggesting that combining multiple parameters may improve the detection of NSTI. The LRINEC score is the most widely evaluated clinical scoring system for detecting NSTIs though its clinical utility in this setting remains controversial. All patients in this study underwent standard-of-care management for suspected NSTI, but not all required lab tests (i.e., C-reactive protein) were conducted to generate complete LRINEC scores for two patients. This finding emphasizes the point that, although the LRINEC score may be a well-studied scoring system, it is not fully implemented clinically. In this study, no statistically significant difference in LRINEC score was observed between NSTI and non-NSTI patient groups (Table 2). If the standard LRINEC score cutoff of had been used herein, the sensitivity and specificity for identifying patients with NSTIs (versus all non-NSTI cases) would have been 67% and 38%, respectively [Fig. 6(a), Table 4]. Meanwhile, if median lesion ROI ICG fluorescence snapshot, IS, or IMAX parameter values had been used, the sensitivity and specificity for NSTI identification would have been 100% and 75%, respectively [Fig. 6(a), Table 4]. If NSTI cases were compared with only cellulitis cases, yielded sensitivity and specificity of 67% and 33%, respectively [Fig. 6(b), Table 4]. Meanwhile, fluorescence snapshot, IS, and IMAX parameter values individually provided perfect discrimination between NSTI and cellulitis cases [Fig. 6(b), Table 4]. This study has limitations that warrant discussion. Only patient-level histopathological data were collected in this study per standard of care. Thus, the spatial distribution of histological markers of infection (e.g., bacterial burden, vascular thrombi) is unknown and cannot be co-registered to spatially localized ICG fluorescence parameters. Future work will involve preclinical modeling of NSTI through which these relationships will be determined in a controlled, laboratory setting. This work will also determine whether ICG fluorescence imaging might be useful for determining the spatial extent of NSTI, thereby providing improved guidance during surgical debridement of affected tissues. ICG perfusion parameters quantified in this study were selected for simplicity and interpretability, but most were defined in terms of arbitrary units (a.u.) of fluorescence. Future work will focus on the quantification of more objective perfusion parameters, such as normalized parameters (e.g., normalized IS)51 and/or parameters defined in terms of absolute units (e.g., radiance) using fluorescence imaging standards. An ICG-equivalent, 3D-printed fluorescence standard was imaged in some cases [e.g., Fig. 2(b)] but not all, precluding its use to standardize fluorescence measurements and enable more quantitative inter-patient/measurement comparisons. Motion artifacts precluded the quantification of additional DCE-FI parameters (e.g., the egress slope) for all patients.41,42 In addition, the commercial imagers used in this study provided a limited dynamic range; all images were compressed to eight-bit (0 to 255 range) data. As such, image saturation occurred in some cases. Finally, our data indicated a potential overlap in ICG fluorescence parameters for patients with diabetes mellitus-associated gangrene, severe osteomyelitis with cutaneous involvement, and NSTI. Future work will focus on improving data collection quality and the use of quantitative, ICG-equivalent fluorescence standards to improve inter-patient/measurement comparisons.52 Furthermore, we anticipate that achieving maximal diagnostic performance may involve clinical parameters (e.g., vitals, labs, imaging) in combination with fluorescence imaging. The results presented herein suggest that ICG fluorescence imaging may be effective for real-time identification of NSTI, expanding the physician’s toolkit for addressing the pressing clinical problem of life-threatening NSTI. Our pilot study is limited in terms of case numbers ( images) and diversity (e.g., skin pigmentation, infection location, and depth in tissue). Additional clinical data collection is warranted to evaluate this fluorescence imaging technique on a larger and more diverse patient cohort. 5.ConclusionNSTIs can be life-threatening, rapidly progressing infections for which accurate, real-time diagnosis strategies currently do not exist. ICG fluorescence imaging for surgical guidance is safe for human use and effective for increasing contrast to critical anatomical structures and pathologic phenomena. Real-time, accurate identification of NSTI may be possible with perfusion-based ICG fluorescence imaging. DisclosuresS.S.S. is a part-time employee of QUEL Imaging LLC, a Small Business Innovation Research-funded start-up that focuses on the commercialization of optical targets for fluorescence-guided surgical systems. J.T.E. is a co-founder of FWD Imaging, Inc., a start-up that focuses on the development of fluorescence-guided debridement technology. E.R.H. is an educational consultant for Stryker Orthopaedics. All other authors declare no competing interests. Code and Data AvailabilityCode and raw image data from this study are available from the corresponding author upon reasonable request. AcknowledgmentsA portion of the data reported herein was included in a 2023 SPIE Proceedings paper (PMCID: PMC10078977). This study was funded by institutional support from the Dartmouth Health Department of Orthopaedics and from the Hitchcock Foundation (Grant No. 250-4141; PI: GSR). We also acknowledge the Necrotizing Fasciitis Research in Orthopedics, Urology, and Surgery (NEFARIOUS) Study Group members for involvement in this project and review of this article: Dr. Scott Rodi, Dr. Jaimo Ahn, Dr. Niels Martin, Dr. Sunil Singhal, Dr. William Obremskey, Dr. Jonathan Shoenecker, Dr. Eben Rosenthal, Dr. Nicholas Bernthal, Dr. Christopher Lee, Dr. Amy Bryant, Dr. Sarah Hobdey, Dr. Dennis Stevens, and Dr. Evan Dellinger. ReferencesF. Nawijn et al.,
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BiographyGabrielle S. Ray is a chief resident physician in orthopedic surgery at Dartmouth Hitchcock Medical Center and has been accepted to the Adult Lower Extremity Reconstructive Surgery Fellowship at the Mayo Clinic beginning in 2025. She earned her BA degree in neuroscience at Colby College and then was a clinical research coordinator for two years at the Harris Orthopaedic Laboratory at Massachusetts General Hospital prior to matriculation at Tufts Medical School. Samuel S. Streeter is a scientist in the Department of Orthopaedics at Dartmouth Hitchcock Medical Center and an assistant professor at the Geisel School of Medicine at Dartmouth. He received his BE and PhD degrees from the Thayer School of Engineering at Dartmouth, specializing in biomedical optics and imaging for surgical guidance applications. |
Fluorescence
Tissues
Fluorescence imaging
Targeting Task Performance metric
Fluorescence intensity
Diagnostics
Imaging systems