Fourier Transform Infrared (FTIR) Studies Of The Lipid Thermotropic Mesomorphism Of Acholeplasma laidlawii B

Hector L. Casal; David G. Cameron; Adele Martin; Henry H. Mantsch

doi:10.1117/12.932159

29 October 1981 Fourier Transform Infrared (FTIR) Studies Of The Lipid Thermotropic Mesomorphism Of Acholeplasma laidlawii B

Hector L. Casal, David G. Cameron, Adele Martin, Henry H. Mantsch

Author Affiliations +

Proceedings Volume 0289, 1981 Intl Conf on Fourier Transform Infrared Spectroscopy; (1981) https://doi.org/10.1117/12.932159
Event: 1981 International Conference on Fourier Transform Infrared Spectroscopy, 1981, Columbia, United States

Abstract

The lipid components of the plasma membrane of the microorganism A. laidlawii B undergo a temperature induced phase transition. The biosynthetic incorporation of deuterated fatty acids into these membranes has allowed, via the C-D stretching vibrations, a detailed study of the lipid organization and dynamics, and the effect of the intrinsic membrane proteins on the transition. A number of fatty acids, of different chain lengths, have been incorporated biosynthetically in the presence or absence of avidin, a fatty acid synthesis inhibitor. In this manner the membranes are enriched solely in the added deuterated fatty acid up to high levels of homogeneity, providing an excellent system for the study of lipid-protein interactions. Detailed data regarding the lipid thermal behaviour will be presented and compared with results obtained by means of other techniques.

Citation Download Citation

Hector L. Casal, David G. Cameron, Adele Martin, and Henry H. Mantsch "Fourier Transform Infrared (FTIR) Studies Of The Lipid Thermotropic Mesomorphism Of Acholeplasma laidlawii B", Proc. SPIE 0289, 1981 Intl Conf on Fourier Transform Infrared Spectroscopy, (29 October 1981); https://doi.org/10.1117/12.932159

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available