Multiphoton excitation of Rose Bengal to induce capillary photo-thrombosis

P. Delafontaine-Martel; Xuecong Lu; Cong Zhang; Rafat Damseh; Frédéric Lesage; Paul J. Marchand

doi:10.1117/12.2577868

5 March 2021 Multiphoton excitation of Rose Bengal to induce capillary photo-thrombosis

P. Delafontaine-Martel, Xuecong Lu, Cong Zhang, Rafat Damseh, Frédéric Lesage, Paul J. Marchand

Proceedings Volume 11629, Optical Techniques in Neurosurgery, Neurophotonics, and Optogenetics; 116290G (2021) https://doi.org/10.1117/12.2577868
Event: SPIE BiOS, 2021, Online Only

Abstract

In this work, we demonstrate the application of Rose Bengal as a photo-thrombotic agent using 2-photon and 3-photon excitation by measuring the response to multiphotonic excitation. We also demonstrate, in the case of 2-photon excitation, that the mechanism used is different from laser injury alone with a control group of FitC injected mice. Preliminary results show that a capillary photo-thrombosis could be performed up to 200 μm and OCT imaging could confirm blockage.

Conference Presentation

Citation Download Citation

P. Delafontaine-Martel, Xuecong Lu, Cong Zhang, Rafat Damseh, Frédéric Lesage, and Paul J. Marchand "Multiphoton excitation of Rose Bengal to induce capillary photo-thrombosis", Proc. SPIE 11629, Optical Techniques in Neurosurgery, Neurophotonics, and Optogenetics, 116290G (5 March 2021); https://doi.org/10.1117/12.2577868

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