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5 March 2021 High-speed volumetric two-photon fluorescence imaging of neurovascular dynamics
Jiang Lan Fan, Jose A. Rivera, Henry Haeberle, Wei Sun, John Peterson, Sam Rubin, Na Ji
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Proceedings Volume 11629, Optical Techniques in Neurosurgery, Neurophotonics, and Optogenetics; 116290J (2021) https://doi.org/10.1117/12.2578636
Event: SPIE BiOS, 2021, Online Only
Abstract
Understanding the structure and function of vasculature in the brain is important for both the interpretation of medical functional imaging data and the mechanistic understanding of neurovascular disorders. Currently, there lacks a volumetric vasculature imaging method able to uncover distributed hemodynamics at sub-capillary resolution and blood flow-resolving speed. Here, we applied two-photon microscopy with an axially extended Bessel focus to fast volumetric imaging of vasculature in the awake brain. We measured vessel dilation, constriction, and 3-dimensional blood flow at unprecedentedly high throughput and capillary resolution. We expect our method to make broad impacts on studying neurovasculature in vivo.
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Jiang Lan Fan, Jose A. Rivera, Henry Haeberle, Wei Sun, John Peterson, Sam Rubin, and Na Ji "High-speed volumetric two-photon fluorescence imaging of neurovascular dynamics", Proc. SPIE 11629, Optical Techniques in Neurosurgery, Neurophotonics, and Optogenetics, 116290J (5 March 2021); https://doi.org/10.1117/12.2578636
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KEYWORDS
Luminescence
Brain
Two photon imaging
Hemodynamics
Blood circulation
Neuroimaging
In vivo imaging
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