Wide-field optical imaging along the neurovascular coupling pathway

Xiaodan Wang; Annie R. Bice; Zachary Rosenthal; Jin-Moo Lee; Adam Q. Bauer

doi:10.1117/12.2577739

5 March 2021 Wide-field optical imaging along the neurovascular coupling pathway

Xiaodan Wang, Annie R. Bice, Zachary Rosenthal, Jin-Moo Lee, Adam Q. Bauer

Proceedings Volume 11629, Optical Techniques in Neurosurgery, Neurophotonics, and Optogenetics; 1162920 (2021) https://doi.org/10.1117/12.2577739
Event: SPIE BiOS, 2021, Online Only

Abstract

Understanding how different diseases of the central nervous system affect neurovascular coupling will allow for linking changes in neural or metabolic dysfunction to changes in hemodynamic signaling upon which blood-based imaging methods rely. We developed a dual fluorophore imaging system for simultaneous, high-speed mapping of neural, metabolic, and hemodynamic activity. Proof-of-concept measurements of spontaneous and stimulus-evoked dynamics are presented in awake and anesthetized mice. This flexible hardware platform allows for integrating optogenetic stimulation for all optical neural circuit interrogation and readout, and for examining the interaction between multiple cell populations.

Conference Presentation

Citation Download Citation

Xiaodan Wang, Annie R. Bice, Zachary Rosenthal, Jin-Moo Lee, and Adam Q. Bauer "Wide-field optical imaging along the neurovascular coupling pathway", Proc. SPIE 11629, Optical Techniques in Neurosurgery, Neurophotonics, and Optogenetics, 1162920 (5 March 2021); https://doi.org/10.1117/12.2577739

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