High-resolution in vivo optical-sectioning widefield microendoscopy

Qinrong Zhang; Daisong Pan; Na Ji

doi:10.1117/12.2578565

5 March 2021 High-resolution in vivo optical-sectioning widefield microendoscopy

Qinrong Zhang, Daisong Pan, Na Ji

Proceedings Volume 11652, Adaptive Optics and Wavefront Control for Biological Systems VII; 1165209 (2021) https://doi.org/10.1117/12.2578565
Event: SPIE BiOS, 2021, Online Only

Abstract

Microendoscopy incorporating a gradient index (GRIN) lens has emerged as a powerful tool for in vivo imaging. The lack of optical sectioning capability of widefield microendoscopy and the intrinsic optical aberrations of the GRIN lens itself, however, limit the achievable image contrast and resolution in three-dimensional (3D) tissues. In this study, we applied HiLo to widefield microendoscopy for optical sectioning. We also utilized adaptive optics (AO) to measure and correct GRIN lens aberrations. Together, HiLo and AO enabled subcellular-resolution microendoscopy imaging with optical sectioning and allowed us to image fine neuronal processes and synapses in the mouse brain in vivo.

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Citation Download Citation

Qinrong Zhang, Daisong Pan, and Na Ji "High-resolution in vivo optical-sectioning widefield microendoscopy", Proc. SPIE 11652, Adaptive Optics and Wavefront Control for Biological Systems VII, 1165209 (5 March 2021); https://doi.org/10.1117/12.2578565

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KEYWORDS

Adaptive optics

In vivo imaging

GRIN lenses

Tissue optics

3D image processing

Luminescence

Tissues

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