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7 March 2023 High resolution mass spectrometry imaging using 3 micron laser ablation
Ronan A. Battle, Daniel Simon, Yuchen Xiang, Kenneth Robinson, Timothy H. Runcorn, Robert T. Murray, J. Roy Taylor, Zoltan Takats
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Proceedings Volume 12377, Optical Interactions with Tissue and Cells XXXIV; 1237706 (2023) https://doi.org/10.1117/12.2649191
Event: SPIE BiOS, 2023, San Francisco, California, United States
Abstract
We report a single-cell level resolution (≤10 µm), laser desorption-based mass spectrometry imaging platform. An optical parametric amplifier is used to generate ∼100 ps, 200 nJ pulses at around 3 µm with a maximum repetition rate of 500 kHz. The pulses are tightly focussed on to fresh frozen animal tissue samples with a thickness of 10 µm. Small volumes of tissue are readily ablated by the laser and are subsequently chemically analyzed using a Rapid Evaporative Ionization Mass Spectrometry (REIMS) source installed on a time of flight mass analyzer. Raster scanning the samples through the laser focus enables the acquisition of mass spectrometry data which can be processed into images with pixel size 10 µm without oversampling, corresponding to cellular level resolution.
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Ronan A. Battle, Daniel Simon, Yuchen Xiang, Kenneth Robinson, Timothy H. Runcorn, Robert T. Murray, J. Roy Taylor, and Zoltan Takats "High resolution mass spectrometry imaging using 3 micron laser ablation", Proc. SPIE 12377, Optical Interactions with Tissue and Cells XXXIV, 1237706 (7 March 2023); https://doi.org/10.1117/12.2649191
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KEYWORDS
Laser ablation
Biological samples
Tissues
Optical parametric amplifiers
Mass spectrometry
Pulsed laser operation
Mid infrared
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