Fast scattering coefficient measurement of intralipid-infused tissue phantoms using imaging sensors

Glenn H. Chapman; Isabella Aguilera; Matthew Schilling

doi:10.1117/12.3003941

12 March 2024 Fast scattering coefficient measurement of intralipid-infused tissue phantoms using imaging sensors

Glenn H. Chapman, Isabella Aguilera, Matthew Schilling

Proceedings Volume 12840, Optical Interactions with Tissue and Cells XXXV; 1284002 (2024) https://doi.org/10.1117/12.3003941
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

The time instability of regular biological tissue makes repeatable optical tomography imaging experiments through tissue difficult, hence the need for long life tissue phantoms with adjustable scattering parameters. Our test phantoms employ a wide density range of intralipid-infused agar layers 1-6 mm thick building phantoms with small to large scattering parameters of values μs =< 20cm^-1, g = 0.95. The intralipid-infused agar is encapsulated within clear polymer stabilizing these for >10 years creating samples with ranges of thicknesses, scattering characteristics and shapes. To tune phantoms we have created an improved rapid measurement method for scattering coefficients μs and anisotropy factor g. Using a DSLR camera with full frame 36x24mm sensor we 3D printed an optical jig which mounts phantoms like a lens to the camera. Aligning laser beams to the phantom a single picture captures ~6 million pixel values over +/-12°, creating 20,000 measurements each at 2300 angular bins of 0.005°. Matlab programs calculate the scattering center and concentric circles of pixels at each angular position. Nonlinear curve fitting the two term Henyey-Greenstein model extracts the pairs of HG parameters: weighing factors, μs and g parameters. Fits are highly statistical significance with exceedingly small deviation from the HG model. Each of 3 test phantom were measured a wide wavelengths range: 405, 532, 632, 670 and NIR 808 nm. The heat mirrors in regular DSLR/Mirror cameras still allow NIR measurements (to ~1000 nm) due to the very low noise of these photography systems.

Conference Presentation

(2024) Published by SPIE. Downloading of the abstract is permitted for personal use only.

Citation Download Citation

Glenn H. Chapman, Isabella Aguilera, and Matthew Schilling "Fast scattering coefficient measurement of intralipid-infused tissue phantoms using imaging sensors", Proc. SPIE 12840, Optical Interactions with Tissue and Cells XXXV, 1284002 (12 March 2024); https://doi.org/10.1117/12.3003941

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