Monitoring the life cycle stages of Haematococcus pluvialis by using FLIM

Yifeng Deng; Changjiang Li; Zeyu Xiao; Jiakai Chen; Zipei Wu; Xiao Peng; Zhen Lu; Wei Yan; Junle Qu

doi:10.1117/12.3023913

18 March 2024 Monitoring the life cycle stages of Haematococcus pluvialis by using FLIM

Yifeng Deng, Changjiang Li, Zeyu Xiao, Jiakai Chen, Zipei Wu, Xiao Peng, Zhen Lu, Wei Yan, Junle Qu

Proceedings Volume 13104, Advanced Fiber Laser Conference (AFL2023); 1310470 (2024) https://doi.org/10.1117/12.3023913
Event: Advanced Fiber Laser Conference (AFL2023), 2023, Shenzhen, China

Abstract

Haematococcus pluvialis is one type of freshwater unicellular microalgae, belonging to the Chlorophyta, Haematococcus. H. pluvialis has high economic value due to its wide variety of nutritional components, including astaxanthin, vitamins, algal polysaccharides, proteins, etc. Current microalgae detection methods are deficient in monitoring the life cycle of H. pluvialis. In this study, we applied the confocal fluorescence microscopy and the fluorescence lifetime imaging microscopy (FLIM) system to observe the different stages of the microalgal cells. The cell morphology and the fluorescence lifetime distributions were obtained for further analysis. The results showed that H. pluvialis had a diameter of about 25μm in the state of flagellated macrozooids (zoospore). When the nutrients in the medium were continuously consumed, the individual cells of H. pluvialis became larger and entered the microzooid stage, whose diameters were around 35μm. The fluorescence lifetime values of H. pluvialis in the macrozooid (zoospore) stage and the microzooid stage were around 700 to 800ps and 1000ps, respectively. The next stage was the palmella stage, in which the diameter of H. pluvialis increased to the range of 40 to 52μm. During this stage, the larger algae began to accumulate red pigment in the center of the cell. Meanwhile, the fluorescence lifetime values near the center of the algal cell increased to around 1300ps, approximately 200ps higher than the values in the periphery of the cells, showing layered properties of H. pluvialis in the palmella stage. In summary, FLIM can provide a real-time quantitative method for monitoring different life stages and cell morphologies of H. pluvialis during its life cycle in a non-destructive way.

(2024) Published by SPIE. Downloading of the abstract is permitted for personal use only.

Citation Download Citation

Yifeng Deng, Changjiang Li, Zeyu Xiao, Jiakai Chen, Zipei Wu, Xiao Peng, Zhen Lu, Wei Yan, and Junle Qu "Monitoring the life cycle stages of Haematococcus pluvialis by using FLIM", Proc. SPIE 13104, Advanced Fiber Laser Conference (AFL2023), 1310470 (18 March 2024); https://doi.org/10.1117/12.3023913

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KEYWORDS

Fluorescence

Fluorescence lifetime imaging

Picosecond phenomena

Confocal microscopy

Pigments

Biological samples

Autofluorescence

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