Optical monitor for microbial metabolism for hazardous waste      application

M. Ryan-Baker; Tuan Vo-Dinh; Guy D. Griffin; Gordon H. Miller; Jean Pierre Alarie; Robert S. Burlage; A. V. Palumbo; Dennis C. White; S. Herbes

doi:10.1117/12.59339

1 May 1992 Optical monitor for microbial metabolism for hazardous waste application

M. Ryan-Baker, Tuan Vo-Dinh, Guy D. Griffin, Gordon H. Miller, Jean Pierre Alarie, Robert S. Burlage, A. V. Palumbo, Dennis C. White, S. Herbes

Author Affiliations +

Proceedings Volume 1637, Environmental and Process Monitoring Technologies; (1992) https://doi.org/10.1117/12.59339
Event: OE/LASE '92, 1992, Los Angeles, CA, United States

Abstract

A remote detection system for in-situ monitoring of the bioluminescence produced by a microbial "reporter" strain grown in rotary film bioreactors has been developed. Two systems were developed: one using a low-temperature photomultiplier (PMT), and one using an avalanche photodiode. The photodiode system was preferred for its sensitivity and portability. Testing was performed on a recombinant constitutive light producing strain. The results indicate that the optical monitor is sensitive enough for direct microbial detection for hazardous waste application.

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M. Ryan-Baker, Tuan Vo-Dinh, Guy D. Griffin, Gordon H. Miller, Jean Pierre Alarie, Robert S. Burlage, A. V. Palumbo, Dennis C. White, and S. Herbes "Optical monitor for microbial metabolism for hazardous waste application", Proc. SPIE 1637, Environmental and Process Monitoring Technologies, (1 May 1992); https://doi.org/10.1117/12.59339

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