Fourier-transform mass spectrometry for high-resolution matrix-assisted laser desorption/ionization MS

John A. Castoro; Charles L. Wilkins

doi:10.1117/12.148521

2 July 1993 Fourier-transform mass spectrometry for high-resolution matrix-assisted laser desorption/ionization MS

John A. Castoro, Charles L. Wilkins

Proceedings Volume 1857, Lasers and Optics for Surface Analysis; (1993) https://doi.org/10.1117/12.148521
Event: OE/LASE'93: Optics, Electro-Optics, and Laser Applications in Scienceand Engineering, 1993, Los Angeles, CA, United States

Abstract

The extended ion trapping time intrinsic to the Fourier transform mass spectrometry (FTMS) experiment allows detection of the extensive fragmentation that can take place during the matrix-assisted laser desorption/ionization process. Metastable ion fragmentation undoubtedly decreases mass resolution for molecular ions in time-of-flight mass spectrometry and, because of the much longer time scale involved, does so even more dramatically in FTMS analysis. However, in this work reported here, it is shown that choice of correct experimental conditions can minimize this problem. For example, for compounds with masses lower than 2000 daltons, mass spectral resolution > 100,000 can be obtained. Spectra of a porphyrin, several proteins and an organic polymer obtained with such resolution are presented. Addition of a sugar co-matrix produced sufficiently stable bovine insulin molecular ions, m/z 5734, to obtain resolution > 25,000.

Citation Download Citation

John A. Castoro and Charles L. Wilkins "Fourier-transform mass spectrometry for high-resolution matrix-assisted laser desorption/ionization MS", Proc. SPIE 1857, Lasers and Optics for Surface Analysis, (2 July 1993); https://doi.org/10.1117/12.148521

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