High-resolution two-photon, three-photon, Harmonic generations and CARS flexible endo-microscope

Vasyl Mytskaniuk; Dylan Septier; Gaelle Brevalle; Victor Tsvirkun; Marc Fabert; Géraud Bouwmans; Frederic Louradour; Alexandre Kudlinski; Hervé Rigneault

doi:10.1117/12.2608999

7 March 2022 High-resolution two-photon, three-photon, Harmonic generations and CARS flexible endo-microscope

Vasyl Mytskaniuk, Dylan Septier, Gaelle Brevalle, Victor Tsvirkun, Marc Fabert, Géraud Bouwmans, Frederic Louradour, Alexandre Kudlinski, Hervé Rigneault

Author Affiliations +

Proceedings Volume PC11937, Endoscopic Microscopy XVII; PC1193705 (2022) https://doi.org/10.1117/12.2608999
Event: SPIE BiOS, 2022, San Francisco, California, United States

Abstract

We present a flexible, compact, 2photon, 3photon, SHG and CARS nonlinear endo-microscope featuring a 2.2 mm outer diameter and a length of 34 mm. It uses a negative curvature hollow-core double-clad fiber that is scanned with a resonant piezo-scanner. The fiber design allows distortion-less, background-free delivery of femtosecond and picosecond excitation pulses and the back-collection of nonlinear signals through the same fiber. Sub-micron spatial resolution together with >300 microns field of view is made possible using micro-lenses or GRIN based miniature objective lens. We demonstrate 2-photon and 3-photon fluorescence, SHG, THG and CARS imaging at a rate of 10 frames/s.

Conference Presentation

Citation Download Citation

Vasyl Mytskaniuk, Dylan Septier, Gaelle Brevalle, Victor Tsvirkun, Marc Fabert, Géraud Bouwmans, Frederic Louradour, Alexandre Kudlinski, and Hervé Rigneault "High-resolution two-photon, three-photon, Harmonic generations and CARS flexible endo-microscope", Proc. SPIE PC11937, Endoscopic Microscopy XVII, PC1193705 (7 March 2022); https://doi.org/10.1117/12.2608999

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