Monitoring the delivery of lipid nanoparticle encapsulated self-amplifying mRNA vaccine using coherent Raman microscopy and multiphoton imaging

Kajari Bera; Prabuddha Mukherjee; Edita Aksamitiene; Aneesh Alex; Jindou Shi; Darold R. Spillman Jr.; Marina Marjanovic; Ahmed Shabana; Russell Johnson; Steve R. Hood; Stephen A. Boppart

doi:10.1117/12.2609087

7 March 2022 Monitoring the delivery of lipid nanoparticle encapsulated self-amplifying mRNA vaccine using coherent Raman microscopy and multiphoton imaging

Kajari Bera, Prabuddha Mukherjee, Edita Aksamitiene, Aneesh Alex, Jindou Shi, Darold R. Spillman Jr., Marina Marjanovic, Ahmed Shabana, Russell Johnson, Steve R. Hood, Stephen A. Boppart

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Proceedings Volume PC11938, Visualizing and Quantifying Drug Distribution in Tissue VI; PC119380A (2022) https://doi.org/10.1117/12.2609087
Event: SPIE BiOS, 2022, San Francisco, California, United States

Abstract

Self-amplifying mRNA (SAM), a synthetic RNA vaccine which self-replicates upon delivery into the cytoplasm encapsulated with lipid nanoparticles (LNPs), leads to a strong and sustained immune response. In this study, we investigated SAM-LNP uptake and subsequent SAM release and distribution in baby hamster kidney (BHK-21) cells using coherent anti-Stokes Raman scattering (CARS) and multiphoton imaging techniques. This work demonstrates the significance of multimodal imaging techniques to capture the successful delivery of SAM and the subsequent production of proteins within cells. Our study can be further extended to label-free detection techniques to investigate targeted drug-delivery.

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Kajari Bera, Prabuddha Mukherjee, Edita Aksamitiene, Aneesh Alex, Jindou Shi, Darold R. Spillman Jr., Marina Marjanovic, Ahmed Shabana, Russell Johnson, Steve R. Hood, and Stephen A. Boppart "Monitoring the delivery of lipid nanoparticle encapsulated self-amplifying mRNA vaccine using coherent Raman microscopy and multiphoton imaging", Proc. SPIE PC11938, Visualizing and Quantifying Drug Distribution in Tissue VI, PC119380A (7 March 2022); https://doi.org/10.1117/12.2609087

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KEYWORDS

Microscopy

Multiphoton microscopy

Nanoparticles

Green fluorescent protein

Raman spectroscopy

CARS tomography

Luminescence

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