Machine learning-assisted label-free multimodal optical bioimaging for biopharmaceutical CHO cell line characterization

Jindou Shi; Alexander Ho; Corey E. Snyder; Eric Chaney; Janet E. Sorrells; Aneesh Alex; Remben Talaban; Darold R. Spillman Jr.; Marina Marjanovic; Steve R. Hood; Stephen A. Boppart

doi:10.1117/12.2648499

6 March 2023 Machine learning-assisted label-free multimodal optical bioimaging for biopharmaceutical CHO cell line characterization

Jindou Shi, Alexander Ho, Corey E. Snyder, Eric Chaney, Janet E. Sorrells, Aneesh Alex, Remben Talaban, Darold R. Spillman Jr., Marina Marjanovic, Steve R. Hood, Stephen A. Boppart

Author Affiliations +

Proceedings Volume PC12371, Multimodal Biomedical Imaging XVIII; PC123710D (2023) https://doi.org/10.1117/12.2648499
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

Label-free multimodal optical bioimaging allows non-perturbative profiling of biological samples based on their intrinsic optical molecular properties. In this study, we utilized SLAM and FLIM microscopy to identify CHO cell lines with favorable process performance for the production of therapeutic monoclonal antibodies and proteins. Here, a single-cell analysis pipeline was developed to quantitatively characterize CHO cell lines based on their phenotypes. To perceive the rich information in the multi-modal bioimages, a custom-built multi-task deep neural network was built, which can extract features from different aspects of the optical and molecular properties of the sample. This work demonstrated the potential of ML-assisted multi-modal optical imaging in the identification of cell lines with desirable characteristics for biopharmaceutical production at earlier time points.

Conference Presentation

Citation Download Citation

Jindou Shi, Alexander Ho, Corey E. Snyder, Eric Chaney, Janet E. Sorrells, Aneesh Alex, Remben Talaban, Darold R. Spillman Jr., Marina Marjanovic, Steve R. Hood, and Stephen A. Boppart "Machine learning-assisted label-free multimodal optical bioimaging for biopharmaceutical CHO cell line characterization", Proc. SPIE PC12371, Multimodal Biomedical Imaging XVIII, PC123710D (6 March 2023); https://doi.org/10.1117/12.2648499

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available