Characterization of Chinese hamster ovary (CHO) cell lines by simultaneous label-free autofluorescence multi-harmonic (SLAM) microscopy for upstream bioprocessing (Conference Presentation)

Alexander Ho; Jindou Shi; Eric Chaney; Corey Snyder; Aneesh Alex; Remben Talaban; Janet Sorrells; Darold R. Spillman Jr.; Marina Marjanovic; Steve R. Hood; Stephen A. Boppart

doi:10.1117/12.2646378

15 March 2023 Characterization of Chinese hamster ovary (CHO) cell lines by simultaneous label-free autofluorescence multi-harmonic (SLAM) microscopy for upstream bioprocessing (Conference Presentation)

Alexander Ho, Jindou Shi, Eric Chaney, Corey Snyder, Aneesh Alex, Remben Talaban, Janet Sorrells, Darold R. Spillman Jr., Marina Marjanovic, Steve R. Hood, Stephen A. Boppart

Author Affiliations +

Proceedings Volume PC12383, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XXI; PC1238302 (2023) https://doi.org/10.1117/12.2646378
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

In the production of biotherapeutics, Chinese hamster ovary (CHO) cells are known as the gold standard. One challenge in the development of these cell lines is the identification of high expressing, yet stable CHO cells. Here we apply simultaneous label-free autofluorescence multi-harmonic (SLAM) microscopy to four CHO cell lines of varying levels of productivity and stability. With the assistance of machine learning, we were able to classify the CHO cell lines into their respective categories with an accuracy of 85%. Application of this CHO cell characterization technology to upstream bioprocessing can potentially improve workflows such as high-throughput screening and monitoring.

Conference Presentation

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Alexander Ho, Jindou Shi, Eric Chaney, Corey Snyder, Aneesh Alex, Remben Talaban, Janet Sorrells, Darold R. Spillman Jr., Marina Marjanovic, Steve R. Hood, and Stephen A. Boppart "Characterization of Chinese hamster ovary (CHO) cell lines by simultaneous label-free autofluorescence multi-harmonic (SLAM) microscopy for upstream bioprocessing (Conference Presentation)", Proc. SPIE PC12383, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XXI, PC1238302 (15 March 2023); https://doi.org/10.1117/12.2646378

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KEYWORDS

Microscopy

Ovary

Image processing

Image segmentation

Luminescence

Manufacturing

Multimodal imaging

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