3D microfluidic biochips made of amorphas fluoropolymer CYTOP using two-photon polymerized molding towards super-resolution bioimaging

Kotaro Obata; Mirai Hanzawa; Felix Sima; Hiroyuki Kawano; Kozunari Ozasa; Yasutaka Hanada; Godai Miyaji; Atsushi Miyawaki; Koji Sugioka

doi:10.1117/12.3002296

13 March 2024 3D microfluidic biochips made of amorphas fluoropolymer CYTOP using two-photon polymerized molding towards super-resolution bioimaging

Kotaro Obata, Mirai Hanzawa, Felix Sima, Hiroyuki Kawano, Kozunari Ozasa, Yasutaka Hanada, Godai Miyaji, Atsushi Miyawaki, Koji Sugioka

Author Affiliations +

Proceedings Volume PC12837, Microfluidics, BioMEMS, and Medical Microsystems XXII; PC1283702 (2024) https://doi.org/10.1117/12.3002296
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

Development of biochips enabling distortion-free imaging in the microchannel filled with a culture medium (water) is required to investigate behavior of living cells in micro and nano environments. Fluoropolymer CYTOP is a promising material as a platform of biochips for the distortion-free imaging, because the refractive index of CYTOP (1.34) is almost same as 1.33 of water. In this study, we have developed a new 3D fabrication method for CYTOP by two-photon polymerized structures using a femtosecond laser as molds, which will be used for fabrication of micro and nano environment platforms for super-resolution bioimaging.

Conference Presentation

Citation Download Citation

Kotaro Obata, Mirai Hanzawa, Felix Sima, Hiroyuki Kawano, Kozunari Ozasa, Yasutaka Hanada, Godai Miyaji, Atsushi Miyawaki, and Koji Sugioka "3D microfluidic biochips made of amorphas fluoropolymer CYTOP using two-photon polymerized molding towards super-resolution bioimaging", Proc. SPIE PC12837, Microfluidics, BioMEMS, and Medical Microsystems XXII, PC1283702 (13 March 2024); https://doi.org/10.1117/12.3002296

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available