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13 March 2024 Optical coherence mechano-microscopy for biomechanical characterization of 3D cancer models
Alireza Mowla, Matt Hepburn, Jiayue Li, Danielle Vahala, Sebastian Amos, Liisa Hirvonen, Rowan Sanderson, Philip Wijesinghe, Samuel Maher, Yu Suk Choi, Brendan Kennedy
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Proceedings Volume PC12844, Optical Elastography and Tissue Biomechanics XI; PC1284406 (2024) https://doi.org/10.1117/12.3005758
Event: SPIE BiOS, 2024, San Francisco, California, United States
Abstract
Solid tumours are typically first diagnosed by palpation, revealing increased stiffness, while cancer cells are usually reported to be softer. The mechanical characteristics are not universal and depend on the cell type and the stage of development. Current techniques are usually point or 2D measurement techniques that lack depth penetration in 3D samples. We are developing optical coherence mechano-microscopy integrated with a confocal fluorescence microscope as a platform to investigate the mechanical phenotypes of 3D cancer models, mimicking the conditions in the native tumour microenvironment. This platform enables unique measurement of the 3D elasticity (i.e. Young’s modulus) of metastatic and non-metastatic breast cancer cell spheroids embedded in GelMAl, presenting the extracellular matrix, co-registered with fluorescence images. Our findings show that cells at the centre of non-metastatic cancer cell spheroids are softer (5.8 kPa) than the cells at the periphery (12.7 kPa). In contrast, migrating cells at the periphery of the metastatic cancer cell spheroids are softer (5.7 kPa) than the less motile cells at the centre of these spheroids (8.0 kPa).
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Alireza Mowla, Matt Hepburn, Jiayue Li, Danielle Vahala, Sebastian Amos, Liisa Hirvonen, Rowan Sanderson, Philip Wijesinghe, Samuel Maher, Yu Suk Choi, and Brendan Kennedy "Optical coherence mechano-microscopy for biomechanical characterization of 3D cancer models", Proc. SPIE PC12844, Optical Elastography and Tissue Biomechanics XI, PC1284406 (13 March 2024); https://doi.org/10.1117/12.3005758
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KEYWORDS
3D modeling
Cancer
Optical coherence
Tumor growth modeling
3D metrology
Biomechanics
Elasticity
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