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13 March 2024 Tomographic behavioral tracking of freely-moving model organisms with a hemispheric Fourier light field microscope
Kevin C. Zhou, Clare Cook, Archan Chakraborty, Joakim Jönsson, Kyung Chul Lee, Xi Yang, Shiqi Xu, Jennifer Bagwell, Donald Fox, Michel Bagnat, Roarke Horstmeyer
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Proceedings Volume PC12848, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXXI; PC128480M (2024) https://doi.org/10.1117/12.3000874
Event: SPIE BiOS, 2024, San Francisco, California, United States
Abstract
It is challenging to study behavior of and track freely-moving model organisms using conventional 3D microscopy techniques. To overcome motion artifacts and prevent the organism from leaving the field of view (FOV), existing techniques require paralyzing or otherwise immobilizing the organism. Here, we demonstrate hemispheric Fourier light field tomography, featuring a parabolic objective that enables synchronized multi-view fluorescence imaging over ~2pi steradians at up to 120 fps and across multi-millimeter 3D FOVs. Our method is not only able to track the 6D pose of freely-moving zebrafish and fruit fly larvae, but also other properties such as heartbeat, fin motion, jaw motion, and muscle contractions. We also demonstrate simultaneous multi-organism imaging.
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Kevin C. Zhou, Clare Cook, Archan Chakraborty, Joakim Jönsson, Kyung Chul Lee, Xi Yang, Shiqi Xu, Jennifer Bagwell, Donald Fox, Michel Bagnat, and Roarke Horstmeyer "Tomographic behavioral tracking of freely-moving model organisms with a hemispheric Fourier light field microscope", Proc. SPIE PC12848, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXXI, PC128480M (13 March 2024); https://doi.org/10.1117/12.3000874
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KEYWORDS
Organisms
Microscopes
Tomography
Algorithm development
3D modeling
3D image processing
3D tracking
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