1 December 2011 Effect of cross-linking with riboflavin and ultraviolet A on the chemical bonds and ultrastructure of human sclera
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J. of Biomedical Optics, 16(12), 125004 (2011). doi:10.1117/1.3662458
Abstract
This study examined the effect of the cross-linking with riboflavin-ultraviolet A (UVA) irradiation on the chemical bonds and ultrastructural changes of human sclera tissues using Raman spectroscopy and atomic force microscopy (AFM). Raman spectroscopy of the normal and cross-linked human sclera tissue revealed different types of the riboflavin-UVA and collagen interactions, which could be identified from their unique peaks, intensity, and shape. Raman spectroscopy can prove to be a powerful tool for examining the chemical bond of collagenous tissues at the molecular level. After riboflavin-UVA treatment, unlike a regular parallel arrangement of normal collagen fibrils, the AFM image revealed interlocking arrangements of collagen fibrils. The observed changes in the surface topography of the collagen fibrils, as well as in their chemical bonds in the sclera tissue, support the formation of interfibrilar cross-links in sclera tissues.
Gyeong-Bok Jung, Ji-Hye Kim, Jin Ik Lim, Samjin Choi, Hun-Kuk Park, Hui-Jae Lee, Kyung-Hyun Jin, "Effect of cross-linking with riboflavin and ultraviolet A on the chemical bonds and ultrastructure of human sclera," Journal of Biomedical Optics 16(12), 125004 (1 December 2011). http://dx.doi.org/10.1117/1.3662458
Submission: Received ; Accepted
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KEYWORDS
Sclera

Collagen

Tissues

Raman spectroscopy

Ultraviolet radiation

Natural surfaces

Atomic force microscopy

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