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5 August 2015 Quantitative phase imaging through scattering media by means of coherence-controlled holographic microscope
Vera Kollarova, Jana Collakova, Zbynek Dostal, Pavel Vesely, Radim Chmelik
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Journal of Biomedical Optics, Vol. 20, Issue 11, 111206 (August 2015). https://doi.org/10.1117/1.JBO.20.11.111206
Abstract
A coherence-controlled holographic microscope (CCHM) enables quantitative phase imaging with coherent as well as incoherent illumination. The low spatially coherent light induces a coherence gating effect, which makes observation of samples possible also through scattering media. The paper describes theoretically and simulates numerically imaging of a two-dimensional object through a static scattering layer by means of CCHM, with the main focus on the quantitative phase imaging quality. The authors have investigated both strongly and weakly scattering media characterized by different amounts of ballistic and diffuse light. It is demonstrated that the phase information can be revealed also for the case of the static, strongly scattering layer. The dependence of the quality of imaging process on the spatial light coherence is demonstrated. The theoretical calculations and numerical simulations are supported by experimental data gained with a model phase object, as well as living carcinoma cells treated in an optically turbid emulsion.
CC BY: © The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.
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Vera Kollarova, Jana Collakova, Zbynek Dostal, Pavel Vesely, and Radim Chmelik "Quantitative phase imaging through scattering media by means of coherence-controlled holographic microscope," Journal of Biomedical Optics 20(11), 111206 (5 August 2015). https://doi.org/10.1117/1.JBO.20.11.111206
Published: 5 August 2015
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Cited by 15 scholarly publications.
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KEYWORDS
Diffusers
Scattering media
Image quality
Phase imaging
Scattering
Light scattering
Spatial coherence
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