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10 September 2015 Calcium neuroimaging in behaving zebrafish larvae using a turn-key light field camera
Carlos Cruz Perez, Antonella Lauri, Panagiotis Symvoulidis, Michele Cappetta, Arne Erdmann, Gil G. Westmeyer
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Journal of Biomedical Optics, Vol. 20, Issue 9, 096009 (September 2015). https://doi.org/10.1117/1.JBO.20.9.096009
Abstract
Reconstructing a three-dimensional scene from multiple simultaneously acquired perspectives (the light field) is an elegant scanless imaging concept that can exceed the temporal resolution of currently available scanning-based imaging methods for capturing fast cellular processes. We tested the performance of commercially available light field cameras on a fluorescent microscopy setup for monitoring calcium activity in the brain of awake and behaving reporter zebrafish larvae. The plenoptic imaging system could volumetrically resolve diverse neuronal response profiles throughout the zebrafish brain upon stimulation with an aversive odorant. Behavioral responses of the reporter fish could be captured simultaneously together with depth-resolved neuronal activity. Overall, our assessment showed that with some optimizations for fluorescence microscopy applications, commercial light field cameras have the potential of becoming an attractive alternative to custom-built systems to accelerate molecular imaging research on cellular dynamics.
CC BY: © The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.
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Carlos Cruz Perez, Antonella Lauri, Panagiotis Symvoulidis, Michele Cappetta, Arne Erdmann, and Gil G. Westmeyer "Calcium neuroimaging in behaving zebrafish larvae using a turn-key light field camera," Journal of Biomedical Optics 20(9), 096009 (10 September 2015). https://doi.org/10.1117/1.JBO.20.9.096009
Published: 10 September 2015
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Cited by 15 scholarly publications and 1 patent.
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KEYWORDS
Cameras
Microscopy
Calcium
Neuroimaging
Brain
Luminescence
Image sensors
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