Improved tumor contrast achieved by single time point dual-reporter fluorescence imaging

Kenneth M. Tichauer; Kimberley S. Samkoe; Kristian J. Sexton; Jason R. Gunn; Tayyaba Hasan; Brian W. Pogue

doi:10.1117/1.JBO.17.6.066001

5 June 2012 Improved tumor contrast achieved by single time point dual-reporter fluorescence imaging

Kenneth M. Tichauer, Kimberley S. Samkoe, Kristian J. Sexton, Jason R. Gunn, Tayyaba Hasan, Brian W. Pogue

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Journal of Biomedical Optics, Vol. 17, Issue 6, 066001 (June 2012). https://doi.org/10.1117/1.JBO.17.6.066001

Abstract

In this study, we demonstrate a method to quantify biomarker expression that uses an exogenous dual-reporter imaging approach to improve tumor signal detection. The uptake of two fluorophores, one nonspecific and one targeted to the epidermal growth factor receptor (EGFR), were imaged at 1 h in three types of xenograft tumors spanning a range of EGFR expression levels (n = 6 in each group). Using this dual-reporter imaging methodology, tumor contrast-to-noise ratio was amplified by >6 times at 1 h postinjection and >2 times at 24 h. Furthermore, by as early as 20 min postinjection, the dual-reporter imaging signal in the tumor correlated significantly with a validated marker of receptor density (P < 0.05, r = 0.93). Dual-reporter imaging can improve sensitivity and specificity over conventional fluorescence imaging in applications such as fluorescence-guided surgery and directly approximates the receptor status of the tumor, a measure that could be used to inform choices of biological therapies.

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Kenneth M. Tichauer, Kimberley S. Samkoe, Kristian J. Sexton, Jason R. Gunn, Tayyaba Hasan, and Brian W. Pogue "Improved tumor contrast achieved by single time point dual-reporter fluorescence imaging," Journal of Biomedical Optics 17(6), 066001 (5 June 2012). https://doi.org/10.1117/1.JBO.17.6.066001

Received: 1 December 2011; Accepted: 11 April 2012; Published: 5 June 2012

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