Optical imaging for brain tissue characterization using relative fluorescence lifetime imaging

Asael Papour; Zachary D. Taylor; Adria J. Sherman; Desiree Sanchez; Gregory Lucey; Linda Liau; Oscar M. Stafsudd; William H. Yong M.D.; Warren S. Grundfest M.D.

doi:10.1117/1.JBO.18.6.060504

12 June 2013 Optical imaging for brain tissue characterization using relative fluorescence lifetime imaging

Asael Papour, Zachary D. Taylor, Adria J. Sherman, Desiree Sanchez, Gregory Lucey, Linda Liau, Oscar M. Stafsudd, William H. Yong M.D., Warren S. Grundfest M.D.

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Journal of Biomedical Optics, Vol. 18, Issue 6, 060504 (June 2013). https://doi.org/10.1117/1.JBO.18.6.060504

Abstract

An autofluorescence lifetime wide-field imaging system that can generate contrast in underlying tissue structures of normal and malignant brain tissue samples with video rate acquisition and processing time is presented. Images of the investigated tissues were acquired with high resolution (∼35 μm ) using an algorithm to produce contrast based on differences in relative lifetimes. Sufficient contrast for delineation was produced without the computation of fluorescence decay times or Laguerre coefficients. The imaged tissues were sent for histological analysis that confirmed the detected imaged tissues morphological findings and correlations between relative lifetime maps and histology identified.

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Asael Papour, Zachary D. Taylor, Adria J. Sherman, Desiree Sanchez, Gregory Lucey, Linda Liau, Oscar M. Stafsudd, William H. Yong M.D., and Warren S. Grundfest M.D. "Optical imaging for brain tissue characterization using relative fluorescence lifetime imaging," Journal of Biomedical Optics 18(6), 060504 (12 June 2013). https://doi.org/10.1117/1.JBO.18.6.060504

Published: 12 June 2013

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