Impact of corpus callosum fiber tract crossing on polarimetric images of human brain histological sections: ex vivo studies in transmission configuration

Deyan Ivanov; Lu Si; Leonard Felger; Theoni Maragkou; Philippe Schucht; Marie-Claire Schanne-Klein; Hui Ma; Razvigor Ossikovski; Tatiana Novikova

doi:10.1117/1.JBO.28.10.102908

12 September 2023 Impact of corpus callosum fiber tract crossing on polarimetric images of human brain histological sections: ex vivo studies in transmission configuration

Deyan Ivanov, Lu Si, Leonard Felger, Theoni Maragkou, Philippe Schucht, Marie-Claire Schanne-Klein, Hui Ma, Razvigor Ossikovski, Tatiana Novikova

Author Affiliations +

Journal of Biomedical Optics, Vol. 28, Issue 10, 102908 (September 2023). https://doi.org/10.1117/1.JBO.28.10.102908

Abstract

Significance

Imaging Mueller polarimetry is capable to trace in-plane orientation of brain fiber tracts by detecting the optical anisotropy of white matter of healthy brain. Brain tumor cells grow chaotically and destroy this anisotropy. Hence, the drop in scalar retardance values and randomization of the azimuth of the optical axis could serve as the optical marker for brain tumor zone delineation.

Aim

The presence of underlying crossing fibers can also affect the values of scalar retardance and the azimuth of the optical axis. We studied and analyzed the impact of fiber crossing on the polarimetric images of thin histological sections of brain corpus callosum.

Approach

We used the transmission Mueller microscope for imaging of two-layered stacks of thin sections of corpus callosum tissue to mimic the overlapping brain fiber tracts with different fiber orientations. The decomposition of the measured Mueller matrices was performed with differential and Lu–Chipman algorithms and completed by the statistical analysis of the maps of scalar retardance, azimuth of the optical axis, and depolarization.

Results

Our results indicate the sensitivity of Mueller polarimetry to different spatial arrangement of brain fiber tracts as seen in the maps of scalar retardance and azimuth of optical axis of two-layered stacks of corpus callosum sections The depolarization varies slightly (<15 % ) with the orientation of the optical axes in both corpus callosum stripes, but its value increases by 2.5 to 3 times with the stack thickness.

Conclusions

The crossing brain fiber tracts measured in transmission induce the drop in values of scalar retardance and randomization of the azimuth of the optical axis at optical path length of 15 μm. It suggests that the presence of nerve fibers crossing within the depth of few microns will be also detected in polarimetric maps of brain white matter measured in reflection configuration.

CC BY: © The Authors. Published by SPIE under a Creative Commons Attribution 4.0 International License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

Citation Download Citation

Deyan Ivanov, Lu Si, Leonard Felger, Theoni Maragkou, Philippe Schucht, Marie-Claire Schanne-Klein, Hui Ma, Razvigor Ossikovski, and Tatiana Novikova "Impact of corpus callosum fiber tract crossing on polarimetric images of human brain histological sections: ex vivo studies in transmission configuration," Journal of Biomedical Optics 28(10), 102908 (12 September 2023). https://doi.org/10.1117/1.JBO.28.10.102908

Received: 10 May 2023; Accepted: 17 August 2023; Published: 12 September 2023

ACCESS THE FULL ARTICLE

JOURNAL ARTICLE
13 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

KEYWORDS

Brain

Polarimetry

Depolarization

Mueller matrices

Neuroimaging

Biological samples

Tumors

Significance

Aim

Approach

Results

Conclusions

Show All Keywords

Keywords/Phrases

Search In:

Publication Years