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29 March 2023 In vivo microstructural investigation of the human tympanic membrane by endoscopic polarization-sensitive optical coherence tomography
Svea Steuer, Joseph Morgenstern, Lars Kirsten, Matthias Bornitz, Marcus Neudert, Edmund Koch, Jonas Golde
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Journal of Biomedical Optics, Vol. 28, Issue 12, 121203 (March 2023). https://doi.org/10.1117/1.JBO.28.12.121203
Abstract

Significance

Endoscopic optical coherence tomography (OCT) is of growing interest for in vivo diagnostics of the tympanic membrane (TM) and the middle ear but generally lacks a tissue-specific contrast.

Aim

To assess the collagen fiber layer within the in vivo TM, an endoscopic imaging method utilizing the polarization changes induced by the birefringent connective tissue was developed.

Approach

An endoscopic swept-source OCT setup was redesigned and extended by a polarization-diverse balanced detection unit. Polarization-sensitive OCT (PS-OCT) data were visualized by a differential Stokes-based processing and the derived local retardation. The left and right ears of a healthy volunteer were examined.

Results

Distinct retardation signals in the annulus region of the TM and near the umbo revealed the layered structure of the TM. Due to the TM’s conical shape and orientation in the ear canal, high incident angles onto the TM’s surface, and low thicknesses compared to the axial resolution limit of the system, other regions of the TM were more difficult to evaluate.

Conclusions

The use of endoscopic PS-OCT is feasible to differentiate birefringent and nonbirefringent tissue of the human TM in vivo. Further investigations on healthy as well as pathologically altered TMs are required to validate the diagnostic potential of this technique.
CC BY: © The Authors. Published by SPIE under a Creative Commons Attribution 4.0 International License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.
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Svea Steuer, Joseph Morgenstern, Lars Kirsten, Matthias Bornitz, Marcus Neudert, Edmund Koch, and Jonas Golde "In vivo microstructural investigation of the human tympanic membrane by endoscopic polarization-sensitive optical coherence tomography," Journal of Biomedical Optics 28(12), 121203 (29 March 2023). https://doi.org/10.1117/1.JBO.28.12.121203
Received: 15 December 2022; Accepted: 27 February 2023; Published: 29 March 2023
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Cited by 2 scholarly publications.
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KEYWORDS
Polarization
Endoscopy
In vivo imaging
Optical coherence tomography
Ear
Tissues
Visualization
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