Fluorescent nanodiamonds (FNDs) have recently developed into an exciting new tool for bioimaging applications. The
material possesses several unique features including high biocompatibility, easy bioconjugation, and perfect
photostability, making it a promising optical nanoprobe in vitro as well as in vivo. This work explores the potential
application of this novel nanomaterial as a photostable, nontoxic tracer in vivo using zebrafish as a model organism. We
introduced FNDs into the yolk of a zebrafish embryo by microinjection at the 1-cell stage. Movements of the injected
particles were investigated by using single particle tracking techniques. We observed unidirectional and stop-and-go
traffic as part of the intricate cytoplasmic movements in the yolk cell. We determined a velocity in the range of 0.19 -
0.40 μm/s for 40 particles moving along with the axial streaming in the early developmental stage (1 to 2 hours post
fertilization) of the zebrafish embryos.
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