Artifacts, nonuniform resolution and a slow reconstruction speed have limited the full capabilities of emerging light-field microscopy for in toto extraction of dynamic spatiotemporal patterns in samples. We overcome this limitation through combining a view-channel-depth (VCD) neural network with light-field microscopy, yielding artifact-free three-dimensional image sequences with uniform spatial resolution and high-video-rate reconstruction throughput. We imaged neuronal activities across moving Caenorhabditis elegans and blood flow in a beating zebrafish heart at single-cell resolution with volumetric imaging rates up to 200 Hz.
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