Dr. Peter B. Yim Profile

Dr. Peter B. Yim

Guest Researcher at National Institute of Standards and Technology

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Publications (4)

Proceedings Article | 12 February 2008 Paper

Thermal properties of gold nanoshells in lipid vesicles studied by single particle tracking measurements

Matthew Clarke, HyeongGon Kang, Peter Yim, Rani Kishore, Kristian Helmerson, Jeeseong Hwang

Proceedings Volume 6849, 68490H (2008) https://doi.org/10.1117/12.766415

KEYWORDS: Gold, Near infrared, Particles, Digital image correlation, Diffusion, Luminescence, Nanoparticles, Microscopy, Temperature metrology, Biomedical optics

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Proceedings Article | 7 February 2007 Paper

Fluorescence intermittency and spectral shifts of single bio-conjugated nanocrystals studied by single molecule confocal fluorescence microscopy and spectroscopy

HyeongGon Kang, Mathew Maye, Dmytro Nykypanchuk, Matthew Clarke, Peter Yim, Jeffrey Krogmeier, Kimberly Briggman, Oleg Gang, Jeeseong Hwang

Proceedings Volume 6430, 643024 (2007) https://doi.org/10.1117/12.713934

KEYWORDS: Luminescence, Confocal microscopy, Molecules, Nanocrystals, Fluorescence spectroscopy, Diffusion, Optical properties, Spectroscopy, Singular optics, Linear filtering

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Proceedings Article | 6 February 2007 Paper

Nanocrystal-based biomimetic system for quantitative flow cytometry

Peter Yim, Marina Dobrovolskaia, HyeongGon Kang, Matthew Clarke, Anil Patri, Jeeseong Hwang

Proceedings Volume 6430, 64301T (2007) https://doi.org/10.1117/12.714138

KEYWORDS: Luminescence, Flow cytometry, Biomimetics, Silica, Standards development, Microscopy, Quantum dots, Sensors, Scattering, Microfluidics

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Flow cytometry has been instrumental in rapid analysis of single cells since the 1970s. One of the common approaches is the immunofluorescence study involving labeling of cells with antibodies conjugated to organic fluorophores. More recently, as the application of flow cytometry extended from simple cell detection to single-cell proteomic analysis, the need of determining the actual number of antigens in a single cell has driven the flow cytomery technique towards a quantitative methodology. However, organic fluorophores are challenging to use as probes for quantitative detection due to the lack of photostability and of quantitative fluorescence standards. National Institute of Standards and Technologies (NIST) provides a set of fluorescein isothiocyanate (FITC) labeled beads, RM 8640, which is the only nationally recognized fluorescent particle standard. On the other hand, optical characteristics of semiconductor nanocrystals or quantum dots or QDs are superior to traditional dye molecules for the use as tags for biological and chemical fluorescent sensors and detectors. Compelling advantages of QDs include long photostability, broad spectral coverage, easy excitation, and suitability for multiplexed sensing. Recently, novel surface coatings have been developed to render QDs water soluble and bio-conjugation ready, leading to their use as fluorescent tags and sensors for a variety of biological applications including immunolabeling of cells. Here, we describe our approach of using fluorescent semiconductor QDs as a novel tool for quantitative flow cytometry detection. Our strategy involves the development of immuno-labeled QD-conjugated silica beads as "biomimetic cells." In addition to flow cytometry, the QD-conjugated silica beads were characterized by fluorescence microscopy to quantitate the number of QDs attached to a single silica bead. Our approach enables flow cytometry analysis to be highly sensitive, quantitative, and encompass a wide dynamic range of fluorescence detection. Quantitative aspects of the proposed flow cytometery-based approach for measurement of the QD-based biomimetic samples are discussed.

Proceedings Article | 29 March 2005 Paper

Single RNA kissing complexes studied by fluorescence resonance energy transfer

Peter Yim, Xiaoyi Zhang, Eric DeJong, Jennifer Carroll, John Marino, Lori Goldner

Proceedings Volume 5699, (2005) https://doi.org/10.1117/12.590896

KEYWORDS: Fluorescence resonance energy transfer, Molecules, Luminescence, Microscopes, Quantum efficiency, Distance measurement, Sensors, Confocal microscopy, Molecular energy transfer, Energy transfer

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