We present the use of the polysaccharide chitosan for immobilizing biomolecules on microfabricated
device surfaces. The main advantages of chitosan are its abundance of primary amine groups and its ability
to be electrodeposited. Biomolecules are easily attached to chitosan's amines by standard glutaraldehyde
chemistry. The electrodeposition of chitosan allows accurate spatial and temporal control of biomolecule
placement. We have used this biofunctionalization approach to develop a biophotonic hybridization sensor.
Here we present for the first time probe DNA functionalization of the chitosan interface and hybridization
detection using fluorescently labeled target DNA and integrated optical waveguides.
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