Injection molded microfluidic devices for biological sample separation and detection

Alfredo M. Morales; Blake A. Simmons; Thomas I. Wallow; K. Jeffery Campbell; Seethambal S. Mani; Brita Mittal; Robert W. Crocker; Eric B. Cummings; Rafael V. Davalos; Linda A. Domeier; Marion C. Hunter; Karen L. Krafcik; Gregory J. McGraw; Bruce P. Mosier; Shane M. Sickafoose

doi:10.1117/12.648135

23 January 2006 Injection molded microfluidic devices for biological sample separation and detection

Alfredo M. Morales, Blake A. Simmons, Thomas I. Wallow, K. Jeffery Campbell, Seethambal S. Mani, Brita Mittal, Robert W. Crocker, Eric B. Cummings, Rafael V. Davalos, Linda A. Domeier, Marion C. Hunter, Karen L. Krafcik, Gregory J. McGraw, Bruce P. Mosier, Shane M. Sickafoose

Author Affiliations +

Proceedings Volume 6109, Micromachining and Microfabrication Process Technology XI; 610901 (2006) https://doi.org/10.1117/12.648135
Event: MOEMS-MEMS 2006 Micro and Nanofabrication, 2006, San Jose, California, United States

Abstract

We are developing a variety of microsystems for the separation and detection of biological samples. At the heart of these systems, inexpensive polymer microfluidic chips carry out sample preparation and analysis. Fabrication of polymer microfluidic chips involves the creation of a master in etched silicon or glass; plating of the master to produce a nickel stamp; large lot chip replication by injection molding; precision chip sealing; and chemical modification of channel surfaces. Separation chips rely on insulator-based dielectrophoresis for the separation of biological particles. Detection chips carry out capillary electrophoresis to detect fluorescent tags that identify specific biological samples. Since the performance and reliability of these microfluidic chips are very sensitive to fluidic impedance, electromagnetic flux, and zeta potential, the microchannel dimensions, shape, and surface chemistry have to be tightly controlled during chip fabrication and use. This paper will present an overview of chip design, fabrication, and testing. Dimensional metrology data, surface chemistry characterization, and chip performance data will be discussed in detail.

Citation Download Citation

Alfredo M. Morales, Blake A. Simmons, Thomas I. Wallow, K. Jeffery Campbell, Seethambal S. Mani, Brita Mittal, Robert W. Crocker, Eric B. Cummings, Rafael V. Davalos, Linda A. Domeier, Marion C. Hunter, Karen L. Krafcik, Gregory J. McGraw, Bruce P. Mosier, and Shane M. Sickafoose "Injection molded microfluidic devices for biological sample separation and detection", Proc. SPIE 6109, Micromachining and Microfabrication Process Technology XI, 610901 (23 January 2006); https://doi.org/10.1117/12.648135

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available