Fluorescence diffuse tomography for detection of RFP-expressed tumors in small animals

Ilya V. Turchin; Alexander P. Savitsky; Vladislav A. Kamensky; Vladimir I. Plehanov; Anna G. Orlova; Mikhail S. Kleshnin; Marina V. Shirmanova; Ilya I. Fix; Vladimir O. Popov

doi:10.1117/12.728470

11 July 2007 Fluorescence diffuse tomography for detection of RFP-expressed tumors in small animals

Ilya V. Turchin, Alexander P. Savitsky, Vladislav A. Kamensky, Vladimir I. Plehanov, Anna G. Orlova, Mikhail S. Kleshnin, Marina V. Shirmanova, Ilya I. Fix, Vladimir O. Popov

Author Affiliations +

Proceedings Volume 6626, Molecular Imaging; 66260R (2007) https://doi.org/10.1117/12.728470
Event: European Conferences on Biomedical Optics, 2007, Munich, Germany

Abstract

Capabilities of tumor detection by different optical methods can be significantly improved by labeling of tumors with fluorescent markers. Creation of tumor cell lines transfected with fluorescent proteins provides the possibility not only to detect tumor, but also to conduct the intravital monitoring studies. Cell lines of human melanomas Mel-P, Mel-Kor and human embryonic kidney HEK-293 Phoenix were transfected with DsRed-Express and Turbo-RFP genes. Emission of RFP in the long-wave optical range permits detection of the deeply located tumors, which is essential for whole-body imaging. Only special tools for turbid media imaging, such as fluorescent diffusion tomography (FDT), enable noninvasive investigation of the internal structure of biological tissue. FDT setup for monitoring of tumor growth in small animals has been created. An animal is scanned in the transilluminative configuration by low-frequency modulated light (1 kHz) from Nd:YAG laser with second harmonic generation at the 532 nm wavelength. An optimizing algorithm for scanning of an experimantal animal is suggested. In vivo experiments were conducted immediately after the subcutaneously injection of fluorescing cells into small animals. It was shown that FDT method allows to detect the presence of fluorescent cells in small animals and can be used for monitoring of tumor growth and anticancer drug responce.

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Ilya V. Turchin, Alexander P. Savitsky, Vladislav A. Kamensky, Vladimir I. Plehanov, Anna G. Orlova, Mikhail S. Kleshnin, Marina V. Shirmanova, Ilya I. Fix, and Vladimir O. Popov "Fluorescence diffuse tomography for detection of RFP-expressed tumors in small animals", Proc. SPIE 6626, Molecular Imaging, 66260R (11 July 2007); https://doi.org/10.1117/12.728470

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KEYWORDS

Tumors

Luminescence

Sensors

3D modeling

Point spread functions

Fluorescent proteins

Tomography

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