Label-free multimodal multiphoton imaging of Liver-on-a-Chip models

Aneesh Alex; Terrence Roh; BanuPriya Sridharan; Jindou Shi; Prabuddha Mukherjee; Eric J. Chaney; James Tunstead; Jan Majer; Kajari Bera; Darold R. Spillman Jr.; Marina Marjanovic; Jason Ekert; Steve R. Hood; Stephen A. Boppart

doi:10.1117/12.2608861

2 March 2022 Label-free multimodal multiphoton imaging of Liver-on-a-Chip models

Aneesh Alex, Terrence Roh, BanuPriya Sridharan, Jindou Shi, Prabuddha Mukherjee, Eric J. Chaney, James Tunstead, Jan Majer, Kajari Bera, Darold R. Spillman Jr., Marina Marjanovic, Jason Ekert, Steve R. Hood, Stephen A. Boppart

Author Affiliations +

Proceedings Volume PC11972, Label-free Biomedical Imaging and Sensing (LBIS) 2022; PC1197202 (2022) https://doi.org/10.1117/12.2608861
Event: SPIE BiOS, 2022, San Francisco, California, United States

Abstract

Liver-on-a-chip is a 3D in vitro hepatic microphysiological system aiming to recreate the conditions of liver tissue on a microscopic scale. CN Bio microphysiological system (CN Bio Innovations, UK) is one of the advanced liver-on-a-chip models. In this study, a multimodal optical imaging platform incorporating nonlinear optical imaging techniques such as multiphoton microscopy (MPM), fluorescence lifetime imaging microscopy (FLIM), coherent anti-Stokes Raman scattering (CARS) microscopy, and simultaneous label-free autofluorescence multiharmonic (SLAM) microscopy was used for characterizing the structural and functional changes associated with inflammation, lipid accumulation and drug uptake in the CNBio liver-on-a-chip model.

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Aneesh Alex, Terrence Roh, BanuPriya Sridharan, Jindou Shi, Prabuddha Mukherjee, Eric J. Chaney, James Tunstead, Jan Majer, Kajari Bera, Darold R. Spillman Jr., Marina Marjanovic, Jason Ekert, Steve R. Hood, and Stephen A. Boppart "Label-free multimodal multiphoton imaging of Liver-on-a-Chip models", Proc. SPIE PC11972, Label-free Biomedical Imaging and Sensing (LBIS) 2022, PC1197202 (2 March 2022); https://doi.org/10.1117/12.2608861

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KEYWORDS

Liver

3D modeling

Multimodal imaging

Multiphoton microscopy

Microscopy

Multiphoton fluorescence microscopy

Fluorescence lifetime imaging

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