In vivo imaging of CRISPRi-attenuated receptor expression by using a miniaturized near-infrared anti-EGFR probe (Conference Presentation)

Alexei A. Bogdanov Jr.; Suresh Gupta; Anand T. N. Kumar; Rahul Pal; Eric Schmidt

doi:10.1117/12.2648483

17 March 2023 In vivo imaging of CRISPRi-attenuated receptor expression by using a miniaturized near-infrared anti-EGFR probe (Conference Presentation)

Alexei A. Bogdanov Jr., Suresh Gupta, Anand T. N. Kumar, Rahul Pal, Eric Schmidt

Author Affiliations +

Proceedings Volume PC12398, Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications XIV; PC123980E (2023) https://doi.org/10.1117/12.2648483
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

We performed synthesis and testing of Fab’-based NIR fluorescent probes with the goal of achieving sufficiently high tumor/background (muscle) ratios at the earlier time points after intravenous injection in comparison to the full-size IgG or larger fragments. Conjugation of NIRDye 800CW to Fab’ resulted in partially quenched imaging probes. Fluorescence intensity imaging of orthotopic xenografts in both EGFR-attenuated and wild-type triple negative breast cancer tumors using Fab’-800CW allowed earlier detection of EGFR expression in vivo.

Conference Presentation

Citation Download Citation

Alexei A. Bogdanov Jr., Suresh Gupta, Anand T. N. Kumar, Rahul Pal, and Eric Schmidt "In vivo imaging of CRISPRi-attenuated receptor expression by using a miniaturized near-infrared anti-EGFR probe (Conference Presentation)", Proc. SPIE PC12398, Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications XIV, PC123980E (17 March 2023); https://doi.org/10.1117/12.2648483

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
PRESENTATION

WATCH
PRESENTATION SAVE TO MY LIBRARY

GET CITATION

KEYWORDS

In vivo imaging

Tumors

Receptors

Cancer

Luminescence

Near infrared

Image registration

Show All Keywords

Keywords/Phrases

Search In:

Publication Years