1 March 2010 Quantitative measurement of reactive oxygen species in vivo by utilizing a novel method: chemiluminescence with an internal fluorescence as reference
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J. of Biomedical Optics, 15(2), 027006 (2010). doi:10.1117/1.3368688
Abstract
Reactive oxygen species (ROS) produced by photodynamic therapy (PDT) is recorded in vivo using a chemiluminescence (CL)-based gated optical system. A novel approach is developed to utilize the fluorescence (FL) of the CL probe as an internal fluorescence to calibrate the observed CL on pharmacokinetics of the probe in situ. The results show that during an in vivo PDT session, the intensity of CL decreases significantly and the decaying of CL is governed by fast and slow time components. By comparing the temporal profile of FL to that of the corresponding CL, it is found that the slow component is mainly attributed to the probe pharmacokinetics, whereas the fast component is likely due to rapid oxygen consumption as a result of PDT treatment. With carefully selected criteria, it is possible to minimize the effect of probe pharmacokinetics. This significantly improves the monitoring method for practical applications.
Yanchun Wei, Da Xing, Shiming Luo, Liyong Yang, Qun Chen, "Quantitative measurement of reactive oxygen species in vivo by utilizing a novel method: chemiluminescence with an internal fluorescence as reference," Journal of Biomedical Optics 15(2), 027006 (1 March 2010). http://dx.doi.org/10.1117/1.3368688
Submission: Received ; Accepted
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KEYWORDS
Photodynamic therapy

Oxygen

In vivo imaging

Luminescence

Chemiluminescence

Tissues

Skin

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