CONFERENCE PROCEEDINGS
Advanced Search
Home > Journals > J. Biomed. Opt. > Volume 19 > Issue 6 > Article
Open Access
11 June 2014 Microscale receiver operating characteristic analysis of micrometastasis recognition using activatable fluorescent probes indicates leukocyte imaging as a critical factor to enhance accuracy
Bryan Q. Spring, Akilan Palanisami, Tayyaba Hasan
Author Affiliations +
Journal of Biomedical Optics, Vol. 19, Issue 6, 066006 (June 2014). https://doi.org/10.1117/1.JBO.19.6.066006
Abstract
Molecular-targeted probes are emerging with applications for optical biopsy of cancer. An underexplored potential clinical use of these probes is to monitor residual cancer micrometastases that escape cytoreductive surgery and chemotherapy. Here, we show that leukocytes, or white blood cells, residing in nontumor tissues—as well as those infiltrating micrometastatic lesions—uptake cancer cell-targeted, activatable immunoconjugates nonspecifically, which limits the accuracy and resolution of micrometastasis recognition using these probes. Receiver operating characteristic analysis of freshly excised tissues from a mouse model of peritoneal carcinomatosis suggests that dual-color imaging, adding an immunostain for leukocytes, offers promise for enabling accurate recognition of single cancer cells. Our results indicate that leukocyte identification improves micrometastasis recognition sensitivity and specificity from 92 to 93%—for multicellular metastases <20 to 30 μm in size—to 98 to 99.9% for resolving metastases as small as a single cell.
© 2014 Society of Photo-Optical Instrumentation Engineers (SPIE) 1083-3668/2014/$25.00 © 2014 SPIE
Citation Download Citation
Bryan Q. Spring, Akilan Palanisami, and Tayyaba Hasan "Microscale receiver operating characteristic analysis of micrometastasis recognition using activatable fluorescent probes indicates leukocyte imaging as a critical factor to enhance accuracy," Journal of Biomedical Optics 19(6), 066006 (11 June 2014). https://doi.org/10.1117/1.JBO.19.6.066006
Published: 11 June 2014
JOURNAL ARTICLE
 5 PAGES
DOWNLOAD PAPER SAVE TO MY LIBRARY
GET CITATION
Lens.org Logo
CITATIONS
Cited by 4 scholarly publications and 2 patents.
Advertisement
Advertisement
RIGHTS & PERMISSIONS
Get copyright permission  Get copyright permission on Copyright Marketplace
KEYWORDS
Cancer
Tumors
Ovarian cancer
Tissues
Luminescence
Receivers
Biological research
RELATED CONTENT
Subscribe to Digital Library
Receive Erratum Email Alert
Back to Top