Design and test of a rigid endomicroscopic system for multimodal imaging and femtosecond laser ablation

Chenting Lai; Matteo Calvarese; Karl Reichwald; Hyeonsoo Bae; Mohammadsadegh Vafaeinezhad; Tobias Meyer-Zedler; Franziska Hoffmann; Anna Mühlig; Tino Eidam; Fabian Stutzki; Bernhard Messerschmidt; Herbert Gross; Michael Schmitt; Orlando Guntinas-Lichius; Jürgen Popp

doi:10.1117/1.JBO.28.6.066004

28 June 2023 Design and test of a rigid endomicroscopic system for multimodal imaging and femtosecond laser ablation

Chenting Lai, Matteo Calvarese, Karl Reichwald, Hyeonsoo Bae, Mohammadsadegh Vafaeinezhad, Tobias Meyer-Zedler, Franziska Hoffmann, Anna Mühlig, Tino Eidam, Fabian Stutzki, Bernhard Messerschmidt, Herbert Gross, Michael Schmitt, Orlando Guntinas-Lichius, Jürgen Popp

Author Affiliations +

Journal of Biomedical Optics, Vol. 28, Issue 6, 066004 (June 2023). https://doi.org/10.1117/1.JBO.28.6.066004

Abstract

Significance

Conventional diagnosis of laryngeal cancer is normally made by a combination of endoscopic examination, a subsequent biopsy, and histopathology, but this requires several days and unnecessary biopsies can increase pathologist workload. Nonlinear imaging implemented through endoscopy can shorten this diagnosis time, and localize the margin of the cancerous area with high resolution.

Aim

Develop a rigid endomicroscope for the head and neck region, aiming for in-vivo multimodal imaging with a large field of view (FOV) and tissue ablation.

Approach

Three nonlinear imaging modalities, which are coherent anti-Stokes Raman scattering, two-photon excitation fluorescence, and second harmonic generation, as well as the single photon fluorescence of indocyanine green, are applied for multimodal endomicroscopic imaging. High-energy femtosecond laser pulses are transmitted for tissue ablation.

Results

This endomicroscopic system consists of two major parts, one is the rigid endomicroscopic tube 250 mm in length and 6 mm in diameter, and the other is the scan-head (10 × 12 × 6 cm³ in size) for quasi-static scanning imaging. The final multimodal image accomplishes a maximum FOV up to 650 μm, and a resolution of 1 μm is achieved over 560 μm FOV. The optics can easily guide sub-picosecond pulses for ablation.

Conclusions

The system exhibits large potential for helping real-time tissue diagnosis in surgery, by providing histological tissue information with a large FOV and high resolution, label-free. By guiding high-energy fs laser pulses, the system is even able to remove suspicious tissue areas, as has been shown for thin tissue sections in this study.

CC BY: © The Authors. Published by SPIE under a Creative Commons Attribution 4.0 International License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

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Chenting Lai, Matteo Calvarese, Karl Reichwald, Hyeonsoo Bae, Mohammadsadegh Vafaeinezhad, Tobias Meyer-Zedler, Franziska Hoffmann, Anna Mühlig, Tino Eidam, Fabian Stutzki, Bernhard Messerschmidt, Herbert Gross, Michael Schmitt, Orlando Guntinas-Lichius, and Jürgen Popp "Design and test of a rigid endomicroscopic system for multimodal imaging and femtosecond laser ablation," Journal of Biomedical Optics 28(6), 066004 (28 June 2023). https://doi.org/10.1117/1.JBO.28.6.066004

Received: 26 March 2023; Accepted: 14 June 2023; Published: 28 June 2023

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