Photobiomodulation (PBM) is a type of phototherapy that uses red or near-infrared (NIR) light to stimulate healing or regeneration of tissues with injury or degradation. In recent years, PBM has been successfully used in the treatment of nervous system diseases. However, the application of optical technology in non-invasive transcranial therapy is greatly limited by the high scattering and absorbance of head tissues including scalp and skull, as well as the transcranial spread properties of light with different parameters still remain unclear. Therefore, for realization of phototherapy for brain diseases, in-depth understanding of the transcranial penetrability of light through head tissues is urgently needed. In this study, the wavelength dependence of light transmittance through scalp and skull of mice was investigated using a transcranial laser at wavelengths of 660 nm or 808 nm, which provides reference for future research and application of transcranial PBM.
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