CONFERENCE PROCEEDINGS
Advanced Search
Home > Journals > J. Biomed. Opt. > Volume 18 > Issue 3 > Article
Open Access
22 November 2012 Analysis of collagen fiber domain organization by Fourier second harmonic generation microscopy
Ara Ghazaryan, Halley F. Tsai, Gor Hayrapetyan, Wei-Liang Chen, Yang-Fang Chen, Myung-Yung Jeong, Chang-Seok Kim, Shean-Jen Chen, Chen-Yuan Dong
Author Affiliations +
Journal of Biomedical Optics, Vol. 18, Issue 3, 031105 (November 2012). https://doi.org/10.1117/1.JBO.18.3.031105
Abstract
We present an automated and systematic two-dimensional discrete Fourier transform (2D-FFT) approach to analyze collagen fiber organization through the use of second harmonic generation (SHG) microscopy. Average orientations of individual domains and Ising-like order parameters introduced to characterize the correlation between orientations of adjacent domains may be used to quantitatively characterize fibrous tissues. Our approach was applied to analyze tissues including rat tail tendon, mouse skin, bovine corneas, and human corneas. We also show that collagen fiber organization in normal and keratokonus human corneas may be distinguished. The current approach may be used for the quantitative differentiation of SHG collagen fiber morphology in different tissues and may be applied for diagnostic purposes.
© The Authors. Published by SPIE under a Creative Commons Attribution 3.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.
Citation Download Citation
Ara Ghazaryan, Halley F. Tsai, Gor Hayrapetyan, Wei-Liang Chen, Yang-Fang Chen, Myung-Yung Jeong, Chang-Seok Kim, Shean-Jen Chen, and Chen-Yuan Dong "Analysis of collagen fiber domain organization by Fourier second harmonic generation microscopy," Journal of Biomedical Optics 18(3), 031105 (22 November 2012). https://doi.org/10.1117/1.JBO.18.3.031105
Published: 22 November 2012
JOURNAL ARTICLE
 6 PAGES
DOWNLOAD PAPER SAVE TO MY LIBRARY
GET CITATION
Lens.org Logo
CITATIONS
Cited by 32 scholarly publications.
Advertisement
Advertisement
RIGHTS & PERMISSIONS
Get copyright permission  Get copyright permission on Copyright Marketplace
KEYWORDS
Tissues
Second-harmonic generation
Cornea
Collagen
Autoregressive models
Harmonic generation
Microscopy
RELATED CONTENT
Subscribe to Digital Library
Receive Erratum Email Alert
Back to Top