Dr. Vladimir P. Zharov Profile

Dr. Vladimir P. Zharov

Nanomedicine Center Director at Univ of Arkansas for Medical Sciences

SPIE Involvement:

Conference Program Committee | Author

Publications (73)

PROCEEDINGS ARTICLE | March 14, 2018

Imaging and navigation of remote controlled nanostructured carriers for theranostics (Conference Presentation)

Proc. SPIE. 10479, Light-Based Diagnosis and Treatment of Infectious Diseases

KEYWORDS: Gold, Nanostructuring, Visualization, Nanoparticles, Optical coherence tomography, Magnetism, Flow cytometry, Navigation systems, Photoacoustic spectroscopy, In vivo imaging

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PROCEEDINGS ARTICLE | April 24, 2017

Towards early in vivo photoacoustic malaria diagnosis with 10,000-fold sensitivity improvement (Conference Presentation)

Proc. SPIE. 10064, Photons Plus Ultrasound: Imaging and Sensing 2017

KEYWORDS: Cancer, Blood, Ultrasonography, Capillaries, Flow cytometry, Transducers, Photoacoustic spectroscopy, In vivo imaging, Signal detection, Animal model studies

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PROCEEDINGS ARTICLE | April 24, 2017

Photoacoustic bio-quantification of graphene based nanomaterials at a single cell level (Conference Presentation)

Proc. SPIE. 10064, Photons Plus Ultrasound: Imaging and Sensing 2017

KEYWORDS: Carbon, Medicine, Agriculture, Tissues, Graphene, Microscopy, Medical research, Nanomaterials, Photoacoustic spectroscopy, Nanomedicine

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PROCEEDINGS ARTICLE | April 19, 2017

Novel in vivo flow cytometry platform for early prognosis of metastatic activity of circulating tumor cells (Conference Presentation)

Proc. SPIE. 10065, Biophotonics and Immune Responses XII

KEYWORDS: Cancer, Tumors, Blood, Toxicity, Flow cytometry, Blood circulation, Cell death, Photoacoustic spectroscopy, In vivo imaging, Nanotherapeutics

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PROCEEDINGS ARTICLE | February 21, 2017

Photoswitchable dye-nanoparticle probes with photothermal switching of light-dark states and colors (Withdrawal Notice)

Proc. SPIE. 10079, Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications IX

KEYWORDS: Near infrared, Proteins, Switches, Biomedical optics, Switching, Nanoparticles, Ultraviolet radiation, Luminescence, Magnetism, Dysprosium, Photoacoustic spectroscopy, Current controlled current source, Absorption

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PROCEEDINGS ARTICLE | March 8, 2013

In vivo detection of circulating tumor cells during tumor manipulation

Proc. SPIE. 8565, Photonic Therapeutics and Diagnostics IX

KEYWORDS: Cancer, Tumors, Surgery, Blood, Skin, Luminescence, Melanoma, Biopsy, Flow cytometry, In vivo imaging

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PROCEEDINGS ARTICLE | March 4, 2013

Photoacoustic monitoring of clot formation during surgery and tumor surgery

Proc. SPIE. 8581, Photons Plus Ultrasound: Imaging and Sensing 2013

KEYWORDS: Laser therapeutics, Cancer, Tumors, Surgery, Blood, Ultrasonography, Blood vessels, Photoacoustic spectroscopy, In vivo imaging, Absorption

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PROCEEDINGS ARTICLE | March 4, 2013

Photoacoustic-fluorescence in vitro flow cytometry for quantification of absorption, scattering and fluorescence properties of the cells

Proc. SPIE. 8581, Photons Plus Ultrasound: Imaging and Sensing 2013

KEYWORDS: Continuous wave operation, Scattering, Nanoparticles, Luminescence, Light scattering, Laser scattering, Flow cytometry, Absorbance, Pulsed laser operation, In vitro testing

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PROCEEDINGS ARTICLE | March 4, 2013

Photoacoustic monitoring of circulating tumor cells released during medical procedures

Proc. SPIE. 8581, Photons Plus Ultrasound: Imaging and Sensing 2013

KEYWORDS: Cancer, Tumors, Surgery, Blood, Blood vessels, Melanoma, Biopsy, Flow cytometry, Photoacoustic spectroscopy, In vivo imaging

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PROCEEDINGS ARTICLE | March 4, 2013

Identification of rolling circulating tumor cells using photoacoustic time-of-flight method

Proc. SPIE. 8581, Photons Plus Ultrasound: Imaging and Sensing 2013

KEYWORDS: Gold, Tumors, Blood, Nanoparticles, Blood vessels, Melanoma, Blood circulation, Ear, Photoacoustic spectroscopy, In vivo imaging

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PROCEEDINGS ARTICLE | May 8, 2012

In-vivo real-time monitoring of nanoparticle clearance rate from blood circulation using high speed flow cytometry

Proc. SPIE. 8427, Biophotonics: Photonic Solutions for Better Health Care III

KEYWORDS: Gold, Blood, Nanoparticles, Ultrasonography, Particles, Flow cytometry, Blood circulation, Photoacoustic spectroscopy, In vivo imaging, Absorption

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SPIE Journal Paper | March 1, 2009

Guest Editorial: Nanophotonics for Diagnostics, Protection, and Treatment of Cancer and Inflammatory Diseases

Tuchin, Valery , Drezek, Rebekah , Nie, Shuming , Zharov, Vladimir

JBO Vol. 14 Issue 02

KEYWORDS: Diagnostics, Cancer, Oncology, Nanophotonics, Imaging spectroscopy, Skin cancer, Nanobiophotonics, Tissues, Particles, Laser scattering

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SPIE Journal Paper | March 1, 2009

In vivo Raman flow cytometry for real-time detection of carbon nanotube kinetics in lymph, blood, and tissues

Biris, Alexandru , Galanzha, Ekaterina , Li, Zhongrui , Mahmood, Meena , Xu, Yang , Zharov, Vladimir

JBO Vol. 14 Issue 02

KEYWORDS: Raman spectroscopy, Lymphatic system, Tissues, In vivo imaging, Ear, Blood, Carbon nanotubes, Cancer, Flow cytometry, Raman scattering

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SPIE Journal Paper | March 1, 2009

Nanophotothermolysis of multiple scattered cancer cells with carbon nanotubes guided by time-resolved infrared thermal imaging

Biris, Alexandru , Boldor, Dorin , Palmer, Jason , Monroe, William , Mahmood, Meena , Dervishi, Enkeleda , Xu, Yang , Li, Zhongrui , Galanzha, Ekaterina , Zharov, Vladimir

JBO Vol. 14 Issue 02

KEYWORDS: Cancer, Thermography, Laser therapeutics, Carbon nanotubes, Infrared imaging, Laser scattering, Tumors, Pulsed laser operation, Radiometry, Temperature metrology

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PROCEEDINGS ARTICLE | February 20, 2008

Threshold parameters of the mechanisms of selective nanophotothermolysis with gold nanoparticles

Proc. SPIE. 6854, Optical Interactions with Tissue and Cells XIX

KEYWORDS: Gold, Proteins, Tissues, Nanoparticles, Particles, Explosives, Laser damage threshold, Pulsed laser operation, Laser tissue interaction, Liquids

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SPIE Journal Paper | September 1, 2007

Photoacoustic flow cytometry: principle and application for real-time detection of circulating single nanoparticles, pathogens, and contrast dyes in vivo

Zharov, Vladimir , Galanzha, Ekaterina , Shashkov, Evgeny , Kim, Jin-Woo , Khlebtsov, Nikolay , Tuchin, Valery

JBO Vol. 12 Issue 05

KEYWORDS: Bacteria, Blood, Nanoparticles, In vivo imaging, Absorption, Blood circulation, Flow cytometry, Photoacoustic spectroscopy, Ear, Near infrared

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SPIE Journal Paper | September 1, 2007

In vivo dynamic light scattering imaging of blood coagulation

Kalchenko, Vyacheslav , Brill, Alexander , Bayewitch, Michael , Fine, Ilya , Zharov, Vladimir , Galanzha, Ekaterina , Tuchin, Valery , Harmelin, Alon

JBO Vol. 12 Issue 05

KEYWORDS: Blood, In vivo imaging, Blood vessels, Ear, Injuries, Dynamic light scattering, Blood circulation, Laser irradiation, Laser scattering, Animal model studies

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PROCEEDINGS ARTICLE | June 22, 2007

Application of gold nanoparticles to x-ray diagnostics and photothermal therapy of cancer

Proc. SPIE. 6536, Saratov Fall Meeting 2006: Coherent Optics of Ordered and Random Media VII

KEYWORDS: Gold, Cancer, Tumors, Tissues, Nanoparticles, Particles, X-rays, Diagnostics, Melanoma, X-ray imaging

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PROCEEDINGS ARTICLE | April 27, 2007

In vivo flow cytometry and time-resolved near-IR angiography and lymphography

Proc. SPIE. 6535, Saratov Fall Meeting 2006: Optical Technologies in Biophysics and Medicine VIII

KEYWORDS: Blood, Light scattering, Blood circulation, Ear, In vivo imaging, Time division multiplexing, Lymphatic system, Plasma, Absorption, Animal model studies

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PROCEEDINGS ARTICLE | February 7, 2007

Advances in intravital microscopy for monitoring cell flow dynamics in vivo

Proc. SPIE. 6436, Complex Dynamics and Fluctuations in Biomedical Photonics IV

KEYWORDS: Biomedical optics, Blood, Blood vessels, Microscopy, Light scattering, Blood circulation, Ear, In vivo imaging, Lymphatic system, Animal model studies

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PROCEEDINGS ARTICLE | February 7, 2007

Laser induced thermal explosion mode for selective nano-photothermolysis of cancer cells

Proc. SPIE. 6436, Complex Dynamics and Fluctuations in Biomedical Photonics IV

KEYWORDS: Gold, Laser therapeutics, Cancer, Nanoparticles, Solids, Laser damage threshold, Tissue optics, Pulsed laser operation, Absorption, Nanorods

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PROCEEDINGS ARTICLE | December 7, 2006

Phototherapeutic technologies for oncology

Proc. SPIE. 5973, Current Research on Laser Use in Oncology: 2000-2004

KEYWORDS: Light emitting diodes, Phototherapy, Oncology, Photodynamic therapy, Cancer, Tumors, Skin, Arteries, Light-emitting diode therapy, Blood circulation

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SPIE Journal Paper | November 1, 2006

Superhigh-sensitivity photothermal monitoring of individual cell response to antitumor drug

Zharov, Vladimir , Galitovsky, Valentin , Lyle, Christopher , Chambers, Timothy

JBO Vol. 11 Issue 06

KEYWORDS: Absorption, Cell death, Pulsed laser operation, Control systems, Laser energy, Laser beam diagnostics, Microscopy, Microscopes, Signal detection, Charge-coupled devices

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SPIE Journal Paper | September 1, 2006

In vivo high-speed imaging of individual cells in fast blood flow

Zharov, Vladimir , Galanzha, Ekaterina , Menyaev, Yulian , Tuchin, Valery

JBO Vol. 11 Issue 05

KEYWORDS: In vivo imaging, High speed imaging, Blood, Blood circulation, Biomedical optics, Animal model studies, In vitro testing, Optical resolution, Light scattering, Absorption

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PROCEEDINGS ARTICLE | July 7, 2006

Optimization of gold nanostructers for laser killing of cancer cells

Proc. SPIE. 6164, Saratov Fall Meeting 2005: Coherent Optics of Ordered and Random Media VI

KEYWORDS: Gold, Plasmons, Laser therapeutics, Optical spheres, Cancer, Nanoparticles, Particles, Spherical lenses, Absorption, Nanorods

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PROCEEDINGS ARTICLE | March 6, 2006

Photoacoustics of individual live cells and particles

Proc. SPIE. 6086, Photons Plus Ultrasound: Imaging and Sensing 2006: The Seventh Conference on Biomedical Thermoacoustics, Optoacoustics, and Acousto-optics

KEYWORDS: Gold, Microscopes, Blood, Nanoparticles, Particles, Resistance, Transducers, In vivo imaging, Acoustics, In vitro testing

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PROCEEDINGS ARTICLE | March 3, 2006

Fluctuation of probe beam in thermolens schematics as potential indicator of cell metabolism, apoptosis, necrosis and laser impact

Proc. SPIE. 6085, Complex Dynamics and Fluctuations in Biomedical Photonics III

KEYWORDS: Statistical analysis, Tissues, Speckle, Scattering, Particles, Light scattering, Biological research, Laser beam diagnostics, Signal detection, Lymphatic system

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PROCEEDINGS ARTICLE | February 24, 2006

Combination of photodynamic and ultrasonic therapy for treatment of infected wounds in animal model

Proc. SPIE. 6087, Biophotonics and Immune Responses

KEYWORDS: Light emitting diodes, Wound healing, Photodynamic therapy, Waveguides, Tissues, Ultrasonics, Light-emitting diode therapy, Microorganisms, Bacteria, Animal model studies

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PROCEEDINGS ARTICLE | February 22, 2006

Combined photovacuum therapy of copulative dysfunction

Proc. SPIE. 6078, Photonic Therapeutics and Diagnostics II

KEYWORDS: Light emitting diodes, Medicine, Phototherapy, Laser therapeutics, Blood, Organisms, Oxygen, Lymphatic system, Instrument modeling, Penis

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SPIE Journal Paper | September 1, 2005

Integrated photothermal flow cytometry in vivo

Zharov, Vladimir , Galanzha, Ekaterina , Tuchin, Valery

JBO Vol. 10 Issue 05

KEYWORDS: Blood, Lymphatic system, In vivo imaging, Laser beam diagnostics, In vitro testing, Absorption, Time division multiplexing, Animal model studies, Blood circulation, Luminescence

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SPIE Journal Paper | September 1, 2005

In vivo integrated flow image cytometry and lymph/blood vessels dynamic microscopy

Galanzha, Ekaterina , Tuchin, Valery , Zharov, Vladimir

JBO Vol. 10 Issue 05

KEYWORDS: Lymphatic system, Time division multiplexing, In vivo imaging, Blood, Image transmission, Blood circulation, Microscopy, Visualization, Velocity measurements, Animal model studies

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SPIE Journal Paper | July 1, 2005

Nanocluster model of photothermal assay: application for high-sensitive monitoring of nicotine-induced changes in metabolism, apoptosis, and necrosis at a cellular level

Zharov, Vladimir , Galitovsky, Valentin , Chowdhury, Parimal

JBO Vol. 10 Issue 04

KEYWORDS: Absorption, Cell death, Pulsed laser operation, Mode conditioning cables, Laser energy, Microscopes, Chromophores, Pancreatic cancer, Luminescence, Image transmission

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PROCEEDINGS ARTICLE | April 25, 2005

Biological detection of low radiation doses with integrated photothermal assay

Proc. SPIE. 5697, Photons Plus Ultrasound: Imaging and Sensing 2005: The Sixth Conference on Biomedical Thermoacoustics, Optoacoustics, and Acousto-optics

KEYWORDS: Cancer, Diagnostics, Transducers, Cell death, Radiation effects, Acoustics, Pulsed laser operation, In vitro testing, Absorption, Mode conditioning cables

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PROCEEDINGS ARTICLE | April 25, 2005

Confocal photothermal flow cytometry in vivo

Proc. SPIE. 5697, Photons Plus Ultrasound: Imaging and Sensing 2005: The Sixth Conference on Biomedical Thermoacoustics, Optoacoustics, and Acousto-optics

KEYWORDS: Gold, Confocal microscopy, Blood, Nanoparticles, In vivo imaging, Time division multiplexing, In vitro testing, Lymphatic system, Absorption, Animal model studies

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PROCEEDINGS ARTICLE | April 15, 2005

Laser-induced synergistic effects around absorbing nanoclusters in live cells

Proc. SPIE. 5695, Optical Interactions with Tissue and Cells XVI

KEYWORDS: Gold, Microscopes, Plasmons, Cancer, Breast cancer, Nanoparticles, Particles, Transmission electron microscopy, Pulsed laser operation, Absorption

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Background and Objective: The application of nanotechnology for laser thermal-based killing of abnormal cells (e.g. cancer cells) targeted with absorbing nanoparticles (e.g. gold solid nanospheres, nanoshells, or rod) is becoming an extensive area of research. We develop an approach to enhance the efficiency of selective nanophotothermolysis of cancer cells through laser-induced synergistic effects around gold nanoparticles aggregated in nanoclusters on cell membrane. Study Design/Materials and Methods: A concept of selective target damages by laser-induced synergistic interaction of optical, thermal, and acoustic fields around clustered nanoparticles is presented with focus on overlapping bubbles from nanoparticles aggregated on cell's membrane. The experimental verification of this concept in vitro was performed by the use a tunable laser pulses (420-570 nm, 8-12 ns, 0.1-300 μJ, laser flux of 0.1-10 J/cm²) for irradiation of MDA-MB-231 breast cancer cells targeted with primary antibodies to which selecttively 40-nm gold nanoparticles were attached by the means of secondary antibodies. The photothermal, electron and atomic force microscopes in combination with viability test (annexin -V-Propidium iodide) were employed to study the nanoparticle's spatial organization, the dynamics of microbubble formations around the particle's clusters, and cells damage. Results: An aggregation of nanoparticles on cell membrane was observed with simultaneous increase bubble formation phenomena, and red-shifted absorption due to plasmon-plasmon resonances into nanoclusters. It led to a significant enhancement, at least two orders of magnitude, of the efficiency of selectively killing cancer cells with nanosecond laser pulses. Conclusion: Described approach allows using relatively small nanoparticles which would be easier delivery to target site with further creation of nanoclusters with larger sizes which provide more profound thermal and related phenomena leading to more efficient laser killing of cancer cells. This nanocluster model might be promising also for treatment or modification different targets (e.g. bacteria, virus, vascular lesions, fat, etc.) as well as teh use different type energy deposition (focused ultrasound, microwave, magnetic field, etc.).

PROCEEDINGS ARTICLE | March 29, 2005

Flow image cytometry in vivo: the capability of high resolution transmission mode

Proc. SPIE. 5696, Complex Dynamics and Fluctuations in Biomedical Photonics II

KEYWORDS: Visualization, Blood, Microscopy, Image resolution, Flow cytometry, Blood circulation, Image transmission, In vivo imaging, Time division multiplexing, Lymphatic system

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PROCEEDINGS ARTICLE | August 20, 2004

Monitoring of small lymphatics function under different impact on animal model by integrated optical imaging

Proc. SPIE. 5474, Saratov Fall Meeting 2003: Optical Technologies in Biophysics and Medicine V

KEYWORDS: Visualization, Tissues, Surgery, Blood, Microscopy, Blood circulation, In vivo imaging, Velocity measurements, Lymphatic system, Animal model studies

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PROCEEDINGS ARTICLE | July 12, 2004

Photothermal imaging of moving cells in lymph and blood flow in vivo

Proc. SPIE. 5320, Photons Plus Ultrasound: Imaging and Sensing

KEYWORDS: Imaging systems, Blood, Microscopy, Blood circulation, In vivo imaging, Velocity measurements, Laser beam diagnostics, Pulsed laser operation, Lymphatic system, Absorption

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PROCEEDINGS ARTICLE | July 12, 2004

Photothermal evaluation of the influence of nicotine, antitumor drugs, and radiation on cellular absorbing structures

Proc. SPIE. 5320, Photons Plus Ultrasound: Imaging and Sensing

KEYWORDS: Cancer, Scattering, Laser energy, Chromophores, Cell death, Laser beam diagnostics, Pulsed laser operation, Environmental sensing, Absorption, Mode conditioning cables

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This short review presents findings from a recent evaluation of the diagnostic capabilities of a new experimental design of the advanced photothermal (PT) imaging system; specifically, its performance in studying the impact of nicotine, a combination of antitumor drugs, and radiation on the absorbing structures of various cells. We used this imaging system to test our hypothesis that low doses of chemicals or drugs lead to changes in cell metabolism, that these changes are accompanied by the shrinking of cellular absorbing zones (e.g. organelles), and that these reactions cause increased local absorption. Conversely, high (toxic) doses may lead to swelling of organelles or release of chromophores into the intracellular space, causing decreased local absorption. In this study, we compared PT images and PT responses of the pancreatic exocrine tumor cell line AR42J resulting from exposure to various concentrations of nicotine versus those of control cells. We found that responses were almost proportional to the drug concentration in concentrations ranging from 1 nM-100 μM, reached saturation at a maximum of approximately 100 μM-1 mM, and then fell rapidly at concentrations ranging from 1-50 mM. We also examined the influence of antitumor drugs (vinblastine and paclitaxel) on KB3 carcinoma cells, with drug concentrations ranging from 10^-10 nM to 10 nM. In this instance, exposure initially led to slight cell activation, which was then followed by decreased cellular PT response. Drug administration led to corresponding changes in the amplitude and spatial intracellular localization of PT responses, including bubble formation, as an indicator of local absorption level. Additionally, it was shown that, depending on cell type, x-ray radiation may produce effects similar to those resulting from exposure to drugs. Independent verification with a combined PT-fluorescence assay and conventional staining kits (trypan blue, Annexin V-propidium iodide [PI]) revealed that this new PT assay has the potential to detect different stages of environmental impact, including changes in cell metabolism and apoptotic- and toxic-related phenomena, at a concentration threshold sensitivity at least three orders of magnitude better than existing assays. This assay may also help optimize combined cancer therapies.

PROCEEDINGS ARTICLE | July 1, 2004

Photothermal guidance for selective photothermolysis with nanoparticles

Proc. SPIE. 5319, Laser Interaction with Tissue and Cells XV

KEYWORDS: Gold, Cancer, Nanoparticles, Laser energy, Particles, Diffusion, Transmission electron microscopy, Pulsed laser operation, Absorption, Bacteria

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PROCEEDINGS ARTICLE | August 27, 2003

Temperature control in interstitial laser cancer immunotherapy

Proc. SPIE. 4961, Laser-Tissue Interaction XIV

KEYWORDS: Infrared detectors, Laser therapeutics, Cancer, Tumors, Tissues, Sensors, Semiconductor lasers, Microwave radiation, Beam controllers, Temperature metrology

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PROCEEDINGS ARTICLE | July 23, 2003

The diagnosis of lymph microcirculation in experimental studies on rat mesentery in vivo

Proc. SPIE. 4965, Optical Diagnostics and Sensing in Biomedicine III

KEYWORDS: Tissues, Imaging systems, Blood, Microscopy, Diagnostics, Transmittance, In vivo imaging, Lymphatic system, Absorption, Animal model studies

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PROCEEDINGS ARTICLE | July 1, 2003

Photothermal microscopy of nano-objects

Proc. SPIE. 4960, Biomedical Optoacoustics IV

KEYWORDS: Gold, Diffraction, Visualization, Microscopy, Particles, Diffusion, Thermal effects, Laser beam diagnostics, Pulsed laser operation, Absorption

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PROCEEDINGS ARTICLE | July 1, 2003

Photothermal tweezers

Proc. SPIE. 4960, Biomedical Optoacoustics IV

KEYWORDS: Optical fibers, Glasses, Particles, Nd:YAG lasers, Carbon dioxide lasers, Fiber lasers, Acoustics, Pulsed laser operation, Liquids, Absorption

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SPIE Journal Paper | July 1, 2002

Photothermal images of live cells in presence of drug

Lapotko, Dmitry , Romanovskaya, Tat'yana , Zharov, Vladimir

JBO Vol. 7 Issue 03

KEYWORDS: Absorption, Pulsed laser operation, Laser beam diagnostics, Chromophores, Control systems, Microscopes, Thermal effects, Refractive index, In vitro testing, Luminescence

PROCEEDINGS ARTICLE | June 10, 2002

Photothermal/microwave radiometry for imaging and temperature feedback

Proc. SPIE. 4618, Biomedical Optoacoustics III

KEYWORDS: Optical fibers, Laser therapeutics, Tissues, Antennas, Radiometry, Spatial resolution, Medium wave, Natural surfaces, Laser tissue interaction, Temperature metrology

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PROCEEDINGS ARTICLE | June 10, 2002

Application of acoustical thermometry to noninvasive monitoring of internal temperature during laser hyperthermia

Proc. SPIE. 4618, Biomedical Optoacoustics III

KEYWORDS: Continuous wave operation, Laser therapeutics, Liver, Optical spheres, Tissues, Transducers, Radiation effects, Pulsed laser operation, Temperature metrology, Absorption

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PROCEEDINGS ARTICLE | June 5, 2002

Photothermal lifetime imaging of cell-drug interactions

Proc. SPIE. 4623, Functional Monitoring and Drug-Tissue Interaction

KEYWORDS: Target detection, Microscopes, Diffraction, Refractive index, Visualization, Diffusion, Chromophores, Laser beam diagnostics, Pulsed laser operation, Absorption

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PROCEEDINGS ARTICLE | May 28, 2002

Photoacoustic tweezers

Proc. SPIE. 4622, Optical Diagnostics of Living Cells V

KEYWORDS: Particles, Fiber lasers, Optical tweezers, Thermal effects, Photoacoustic spectroscopy, Convection, Acoustics, Pulsed laser operation, Liquids, Absorption

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PROCEEDINGS ARTICLE | May 23, 2002

Development imaging and experimental model for studying pathogenesis and treatment efficacy of postmastectomy lymphedema

Proc. SPIE. 4624, Optical Diagnostics and Sensing of Biological Fluids and Glucose and Cholesterol Monitoring II

KEYWORDS: Veins, Data modeling, Tissues, Blood, Blood vessels, Microscopy, Pathogens, Blood circulation, Lymphatic system, Animal model studies

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PROCEEDINGS ARTICLE | September 28, 2001

Autonomous microdevices for phototherapy

Proc. SPIE. 4560, Microfluidics and BioMEMS

KEYWORDS: Light emitting diodes, Phototherapy, Blood, Organisms, Mouth, Microorganisms, Pathogens, Intestine, Lymphatic system, Clinical trials

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PROCEEDINGS ARTICLE | July 9, 2001

Photo-pharmaceutical therapy: features and prospects

Proc. SPIE. 4257, Laser-Tissue Interaction XII: Photochemical, Photothermal, and Photomechanical

KEYWORDS: Magnesium, Phototherapy, Laser therapeutics, Photodynamic therapy, Tumors, Blood, Skin, Luminescence, Ultrasonics, Lymphoma

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PROCEEDINGS ARTICLE | July 9, 2001

Combined interstitial laser therapy for cancer using microwave radiometric sensor and RODEO MRI feedback: I. Radio microwave

Proc. SPIE. 4257, Laser-Tissue Interaction XII: Photochemical, Photothermal, and Photomechanical

KEYWORDS: Breast, Laser therapeutics, Cancer, Tumors, Tissues, Skin, Microwave radiation, Microwave radiometry, Body temperature, Temperature metrology

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PROCEEDINGS ARTICLE | July 9, 2001

Deep penetration of light into biotissue

Proc. SPIE. 4257, Laser-Tissue Interaction XII: Photochemical, Photothermal, and Photomechanical

KEYWORDS: Optical fibers, Light sources, Eye, Tissues, Abdomen, Monte Carlo methods, Tissue optics, Uterus, Laparoscopy, Fetus

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PROCEEDINGS ARTICLE | June 15, 2001

Photothermal modification of optical microscope for noninvasive living cell monitoring

Proc. SPIE. 4256, Biomedical Optoacoustics II

KEYWORDS: Microscopes, Optical microscopes, Sensors, Microscopy, Chromophores, Temporal resolution, Spatial resolution, Laser beam diagnostics, Pulsed laser operation, Absorption

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Photothermal method was applied to improve sensing and imaging capabilities of a light microscope in cell studies. We describe the methods, technical details and testing results of cytometric application of Laser Photothermal Phase Microscope (LPPM). The merits of the proposed approach include living single cell monitoring capability, quantitative measurement of cell functional features through the use of cell natural chromophores as the sensors. Such intracellular sensors are activated by the laser pulse and transform an absorbed energy into the heat. The latter causes thermal and mechanical loads to a cell and its components. The second stage of the process includes the reaction of the cell as integral system or of its components to such loads. This reaction is caused by the changes of cell functional and structural state and includes alterations of cell optical properties. Both processes are monitored for a single cell non-invasively with probe laser beam. Pulsed phase contrast dual beam illumination scheme with acquisition of several laser images at different stages of cell-laser interaction was introduced. An acquired cell image is considered as spatially and temporally resolved cell response to non-specific load that is induced in a cell with a pump laser. This method eliminates any cell staining and allows to monitor cell viability and cell reaction to the environmental factors. Also LPPM offers further improvement of spatial and temporal resolution of optical microscope: with pulsed probe laser monitoring we can detect components with the size down to 50 nm and temporal resolution of 10 ns. In our set up the cell is pumped by pulsed laser at 532 nm, 10 ns , 0.01-0.4 mJ. The source of probe beam is a pulsed dye laser (630 nm, 10 nJ, 10 ns) which forms cell phase image. The results obtained with living cells such as drug impact control, single cell dosimetry, immune action of light on a cell demonstrate basic features of LPPM as the tool for the study of the spectral and spatial low-absorption distribution in living cells in addition to techniques of active thermal probing of sub-micron, low-absorbing samples.

PROCEEDINGS ARTICLE | May 21, 2001

Phototherapeutic treatment of patients with peripheral nervous system diseases by means of LED arrays

Proc. SPIE. 4244, Lasers in Surgery: Advanced Characterization, Therapeutics, and Systems XI

KEYWORDS: Light emitting diodes, Phototherapy, Breast cancer, Tissues, Pathology, Arteries, Radiotherapy, Blood circulation, Nervous system, Lymphatic system

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PROCEEDINGS ARTICLE | May 21, 2001

Combined photoultrasonic treatment of infected wounds

Proc. SPIE. 4244, Lasers in Surgery: Advanced Characterization, Therapeutics, and Systems XI

KEYWORDS: Light emitting diodes, Photodynamic therapy, Waveguides, Tissues, Surgery, Capillaries, Ultrasonics, Inflammation, Microorganisms, Therapeutics

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PROCEEDINGS ARTICLE | May 21, 2001

Laser-irradiated drug chromatographic analysis and laser injection of drugs to treat staphyloccocal lesions of skin

Proc. SPIE. 4244, Lasers in Surgery: Advanced Characterization, Therapeutics, and Systems XI

KEYWORDS: Magnesium, Laser therapeutics, Statistical analysis, Skin, Laser energy, Laser development, Laser irradiation, Er:YAG lasers, Pulsed laser operation, Chromatography

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PROCEEDINGS ARTICLE | June 13, 2000

Light activation of the immune system part I: influence on G-class antibodies

Proc. SPIE. 3914, Laser-Tissue Interaction XI: Photochemical, Photothermal, and Photomechanical

KEYWORDS: Optical filters, Proteins, Light emitting diodes, Photodynamic therapy, Patents, Blood, Lamps, Pathogens, Plasma, Absorption

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PROCEEDINGS ARTICLE | June 13, 2000

Photodynamic modulation of immune properties of blood cells

Proc. SPIE. 3914, Laser-Tissue Interaction XI: Photochemical, Photothermal, and Photomechanical

KEYWORDS: Laser therapeutics, Photodynamic therapy, Modulation, Blood, Chromophores, Microorganisms, Picosecond phenomena, Radiation effects, In vitro testing, Bacteria

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PROCEEDINGS ARTICLE | May 19, 2000

Comparison possibilities of ultrasound and its combination with laser in surgery and therapy

Proc. SPIE. 3916, Biomedical Optoacoustics

KEYWORDS: Laser therapeutics, Tumors, Tissues, Surgery, Ultrasonography, Skin, Laser applications, Ultrasonics, Cavitation, Acoustics

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PROCEEDINGS ARTICLE | May 19, 2000

Role of photoacoustic effects occurring at laser perforation of skin and laser transcutaneous drug delivery

Proc. SPIE. 3916, Biomedical Optoacoustics

KEYWORDS: Quartz, Skin, Interfaces, Laser applications, Wave plates, Er:YAG lasers, Photoacoustic spectroscopy, Signal detection, Pulsed laser operation, Absorption

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PROCEEDINGS ARTICLE | May 17, 2000

Photomatrix LED therapy of extensive cutaneous pathology

Proc. SPIE. 3907, Lasers in Surgery: Advanced Characterization, Therapeutics, and Systems X

KEYWORDS: Light emitting diodes, Phototherapy, Laser therapeutics, Photodynamic therapy, Cancer, Tumors, Tissues, Blood, Pathology, Light-emitting diode therapy

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PROCEEDINGS ARTICLE | May 17, 2000

Transurethral electrolaser complex therapy to treat chronic prostatitis

Proc. SPIE. 3907, Lasers in Surgery: Advanced Characterization, Therapeutics, and Systems X

KEYWORDS: Laser therapeutics, Tissues, Sensors, Blood, Electrodes, Magnetism, Infrared lasers, Inflammation, Blood circulation, Prostate

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According to the world statistics, from 30 to 60 percent of elderly male population suffer from chronic prostatitis in different countries. This disease has a number of consequences such as urino-genital inflammation, dysuria, perineal pain, reduction in the physiological activity of smooth muscles, blockage of the anus passages with micro-organism vital activity products, appearance of stagnant zones and low blood circulation complicated by disorders of the sexual function. Most of these features make it difficult to use standard drug therapies with antibiotics or immunocorrectors. For that reason, the objective of this study is to develop and to investigate a novel combined electrolaser therapy which improves drug delivery in the prostate gland and simultaneously provides an independent physiotherapeutic effect. The main feature of this therapy is the utilization of two diode lasers emitting in the red (0.67 micrometer, 10 mW) and in the infrared (0.85 micrometer, 1 W) spectrum ranges in combination with transurethral electrostimulation. An electrolaser catheter containing both hollow cylindrical electrodes and an axial optical fiber to deliver laser radiation was brought along the urethra to the seminal vesicles. The red laser in combination with a photosensitizer ('Photosens,' Russia) was used to realize the antibacterial treatment of the urethra. The infrared laser was employed to heat the prostate gland and to stimulate the blood perfusion without thermal damage of tissues. The laser heating of the prostate at a local tissue temperature of 41 degrees Celsius in combination with the electrostimulation provided approximately a 4.5-fold increase in the blood flow. The realization of an additional mode of photovacuum therapy inside the urethra together with the electrostimulation made it possible to 'clean' the anus passages and to improve the DNA diagnosis reliability in respect of the urogenital infectious remainder. The clinical data obtained in 980 patients, who had been undergoing the treatment since 1994 till 1999 in accordance with the electrolaser method in Russia, are analyzed.

PROCEEDINGS ARTICLE | May 17, 2000

Phototherapeutic treatment of lymphedema and other complications after mastectomy

Proc. SPIE. 3907, Lasers in Surgery: Advanced Characterization, Therapeutics, and Systems X

KEYWORDS: Light emitting diodes, Phototherapy, Laser therapeutics, Tissues, Surgery, Blood, Skin, Arteries, Blood circulation, Lymphatic system

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PROCEEDINGS ARTICLE | March 15, 2000

Optoelectronic microdevices for combined phototherapy

Proc. SPIE. 3912, Micro- and Nanotechnology for Biomedical and Environmental Applications

KEYWORDS: Light emitting diodes, Phototherapy, Nerve, Mouth, Magnetism, Receivers, Power supplies, Optoelectronics, Inductance, Clinical trials

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PROCEEDINGS ARTICLE | December 30, 1999

Laser hybrid and phototherapeutic technologies for oncology

Proc. SPIE. 4059, Laser Use in Oncology II

KEYWORDS: Light emitting diodes, Oncology, Photodynamic therapy, Tumors, Tissues, Blood, Skin, Lamps, Bone, Laser cutting

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PROCEEDINGS ARTICLE | July 14, 1999

Photomedicine with laser drug delivery technologies

Proc. SPIE. 3829, ALT'98 Selected Papers on Novel Laser Methods in Medicine and Biology

KEYWORDS: Laser therapeutics, Skin, Diffusion, Laser applications, Semiconductor lasers, Microorganisms, Diodes, Pulsed laser operation, Erbium lasers, Bacteria

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PROCEEDINGS ARTICLE | July 6, 1999

Photothermal microscopy study of photodynamic inactivation of bacteria in the presence of living blood cells

Proc. SPIE. 3592, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy VIII

KEYWORDS: Blood, Microscopy, Bacteria

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PROCEEDINGS ARTICLE | June 22, 1999

Laser-ultrasonic technologies for medicine

Proc. SPIE. 3590, Lasers in Surgery: Advanced Characterization, Therapeutics, and Systems IX

KEYWORDS: Optical fibers, Transformers, Laser therapeutics, Surgery, Ultrasonography, Laser applications, Laser development, Prostate, Laser cutting, Acoustics

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PROCEEDINGS ARTICLE | July 28, 1994

Transmission of laser radiation and acoustical waves via optical fibers for surgical therapy

Proc. SPIE. 2131, Biomedical Fiber Optic Instrumentation

KEYWORDS: Optical fibers, Laser therapeutics, Silica, Tissues, Ultrasonography, Metals, Glasses, Fiber lasers, Transducers, Plasma

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PROCEEDINGS ARTICLE | February 1, 1994

Acoustical waves via optical fibers for biomedical applications

Proc. SPIE. 2084, Biomedical Optoelectronic Devices and Systems

KEYWORDS: Optical fibers, Laser therapeutics, Biomedical optics, Silica, Tissues, Ultrasonography, Glasses, Coating, Endoscopes, Laser cutting

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PROCEEDINGS ARTICLE | July 7, 1993

New technology in surgery: combination of laser and ultrasound

Proc. SPIE. 1892, Medical Lasers and Systems II

KEYWORDS: Optical fibers, Tissues, Surgery, Ultrasonography, Laser applications, Fiber lasers, Ultrasonics, Bone, Laser cutting, Laser tissue interaction

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